CELL ROOM TEMPERATURE STABILIZATION SOLUTION
Discover a smarter way to preserve cells at room temperature and say goodbye to the -80ºC freezers. 300K Cell Stabilization Solution ensures unmatched reliability and efficiency for your genomic research. It is optimized for White Blood Cells, Buffy coat and Cell lines.
Perfect Preservation. No Freezing Needed!
Room temperature storage keeps cells DNA intact and ready for analysis—no freezers, no degradation risks.
Optimize your space
Store more samples in less space by keeping them at room temperature and free up valuable freezer space.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality
Versatile Applications
Applicable for white blood cells, buffy coat and cell lines. DNA remains viable for high-level analyses.
Shipping has never been this simple.
Transport samples easily safely at room temperature and avoid the risks of cold chain logistics.
Reference Material
Improve standardisation and achieve reproducible results by using cell lines to create reference materials at room temperature.
PRODUCTS
HOW IT WORKS?
The 300K Room Temperature Stabilization Solution is a technology based on the lyophilization of biological samples in a uniform and standardized process. By removing nearly all moisture, it halts degradation processes and allows samples to be maintained at room temperature while preserving their integrity after rehydration. This process requires two key elements: the Sample Stabilization System (S³), a preset freeze-drying device developed by 300K to ensure optimal and reproducible conditions, and the Sample Stabilization Kits, which includes vials pre-coated with specific excipients that protect the sample during lyophilization without interfering in downstream analyses—so no washing is needed after rehydration. This combined system ensures reliable and ready-to-use stabilization, much more than a storage tube.
The DNA extracted from samples freeze-dried using 300K Cell Solution has been subjected torigorous quality control to assess DNA purity, integrity and functionality room temperature storage,following the Proficiency Standards established by the International Society for Biological andEnvironmental Repositories (ISBER).
DNA quality in White Blood Cells
DNA purity and integrity
DNA was extracted from white blood cells preserved at room temperature and from conventionally frozen controls. QC of DNA extracted from cell samples freeze-dried with 300K Cell Stabilization kit showed good purity and integrity when assessed by spectrophotometry and agarose gelelectrophoresis, respectively.
| Sample | Concentration (ng/uL) | A260/280 | A260/230 | [dsDNA] |
|---|---|---|---|---|
| Freeze-dried 1 | 261.831 | 1.846 | 2.435 | 195.0 |
| Freeze-dried 2 | 249.939 | 1.835 | 2.416 | 189.5 |
| Freeze-dried 3 | 239.459 | 1.846 | 2.426 | 167.5 |
| Frozen 1 | 252.426 | 1.849 | 2.375 | 200.0 |
| Frozen 2 | 54.610 | 1.881 | 1.392 | 24.5 |
| Frozen 3 | 179.586 | 1.850 | 2.336 | 111.5 |
Table. Measurement of DNA purity and quantity with spectrophotometry (using Nanodrop OneC).
Figure. DNA integrity assessed by agarose gel electrophoresis.
DNA integrity and functionality
Moreover, we additionally performed a multiplex long PCR, and all samples displayed a 17.5 kb band, confirming that our technology has no negative effect on DNA functionality after RT storage.
Figure. DNA integrity and functionality assessed by multiplex long PCR.
DNA quality in Cell Lines
We assessed DNA quality extracted from 4 different lymphoproliferative disorders (LPDs) cell lines (H929, CA46, RS4;11 and REH) by spectrophotometry and agarose gel electrophoresis.
DNA purity and yield
DNA purity was assessed by spectrophotometry revealing that all four cell lines showed A260/280 ratio values above 1.6 even after 3 months of room temperature (RT) storage, which indicates absence of protein contamination.
Moreover, double-strand DNA quantification by fluorimetry confirmed a good DNA yield in all samples (at least 0.5 µg).
| Sample ID | Time of RT storage | DNA yield (µg) | A260/280 |
|---|---|---|---|
| H929 | Control (T0) | 1.65 | 1.99 |
| 3 months | 2.76 | 1.93 | |
| CA46 | Control (T0) | 1.42 | 1.97 |
| 3 months | 2.59 | 1.88 | |
| RS4;11 | Control (T0) | 0.52 | 1.92 |
| 3 months | 0.94 | 1.81 | |
| REH | Control (T0) | 2.97 | 1.93 |
| 3 months | 1.75 | 1.86 |
Table. DNA yield and purity (A260/280 ratio) for four cell lines stored at room temperature for 3 months.
DNA Functionality and integrity
Using agarose gel electrophoresis, we confirmed DNA integrity in all four cell lines (A), which we further assessed together with DNA functionality, by multiplex long PCR. All samples displayed a 17.5 kb band (B), even after 3 months of RT storage.
Figure. DNA integrity (A) and functionality (B) assessed by agarose gel electrophoresis and multiplex long PCR after 3 months of RT storage.
At 300K Solutions, we validate the compatibility of our stabilized samples with different downstream applications.
Suitability for NGS studies
Specifically, we performed the EuroClonality-NDC assay (Univ8 Genomics, Belfast, North Ireland) which is used for the study of Lymphoproliferative disorders (LPD). The reason why we choose this genomic assay is because it uses as validation samples, among others, the four cell lines we have tested.
Figure. Workflow for NGS analysis of DNA from four cell lines stored at room temperature for 1 year.
The metrics obtained in this NGS analysis using DNA from all four cell lines (stored 1 year at RT) were within the stablished acceptance criteria:
- 17/17 (100%) expected IG rearrangements were detected across the 4 cell lines
- 10/10 (100%) expected TCR rearrangements were detected across the 4 cell lines.
- The correct structural variant was detected in each cell line and confirmed using IGV analysis of the BAM file (see table below)
- All expected mutations (n=29) were reported with good agreement in reported variant allele frequency
| Cell Line | Expected Translocation | Detected |
|---|---|---|
| CA46 | t(8;14) MYC/IGHswc | Yes |
| H929 | t(4;14) MMSET/IGHswc | Yes |
| H929 | t(8;20) MYC/FAM242A | Yes |
| REH | t(1;2) HYDIN2/IGKV | Yes |
| RS4;11 | t(4;11) AFF1/KMT2A | Yes |
Table. Expected translocations and detection results for four cell lines analyzed by NGS.
Valid for Allele Specific Real-Time Quantitative PCR (ASO-qPCR)
We performed a study with the KIT D816V mutation, characteristic of the mastocytosis cell line HMC1, comparing one fresh sample with three lyophilized samples. Dilutions were prepared to obtain a 10% frequency of the mutation, and since the mutation is present in only one allele, the expected frequency of detection was 5%.
Figure. Workflow for qPCR analysis in fresh and lyophilized samples.
The results showed that the fresh sample had 5.01% mutated cells, while the lyophilized samples gave values of 5.22%, 5.40% and 6.65%, with a deviation of 30% (range 3.5% – 6.5%) considered acceptable. This confirmed that 2 of the 3 lyophilized samples were within the expected limits, demonstrating that our stabilization technology allows samples to be stored at room temperature without compromising the accuracy of genetic analyses and ensuring their reliability in downstream applications such as ASO-qPCR.
| Sample | Result | Mutant cells |
|---|---|---|
| Fresh | Positive | 5.01% |
| Lyo. 1 | Positive | 5.22% |
| Lyo. 2 | Positive | 5.40% |
| Lyo. 3 | Positive | 6.65% |
Table. qPCR results and percentage of mutated cells in fresh and lyophilized samples.
