CellShip Cell Transport Medium insulin-free with antibiotics
Xeno-free Storage and Transport Media without insulin and with antibiotic (Penicillin-Streptomycin-Amphotericin)
CELLSHIP® is a fully defined, xeno-free cell transportation medium. A new alternative to cryopreservation for the transport and storage of mammalian cell lines. With this product, cells can be transported between sites at ambient temperature providing an innovative alternative to logistically challenging and expensive dry ice or shippers.
Features
CELLSHIP® has been developed to provide an alternative to cryopreservation for the transport and short-term storage of mammalian cells. Cells can be transported between sites at ambient temperature providing an innovative alternativa to logistically challenging and expensive dry ice or shippers.
The protocol requires miniaml cell manipulation, reducing the risk of contamination and improving efficiency within the laboratory.
Main product features:
– Sterile and Xeno-free
– Non-toxic additives
– Protection against shear stress and maintains membrane integrity
– Ambient temperature
– Insuline-free
– With antibiotics
Specifications
| Specification | Details |
|---|---|
| Available Sizes | 100 mL |
| Formulation | Xeno-free, solvent-free |
| Use | Transport and short term storage |
| pH | pH 7.20 – 7.45 for temperatures between + 2 ºC to + 37 ºC |
| Shelf life | 10 months from date of manufacture |
| Storage | + 2 ºC to + 8 ºC |
| Shipping | Ambient temperature |
| Available antibiotics | Penicillin-Streptomycin and Amphotericin |
Advantages of CellShip®
• Ready-to-use
• Flexibility to highly time critical processes.
• Removes the cost and logistic challenges associated with shipment on dry ice.
• Stable pH-buffering of pH 7.20 – 7.45 for temperatures between +2°C to +37°C.
• Endotoxin levels conform to EU standards (≤ 1.0 EU/mL).
• Solvent-free i.e., free from DMSO.
• Manufactured under ISO 13485.
HepG2 cells were imaged following a 48h recovery period. Prior to recovery, cells had been transported/stored for 72h either cryopreserved in dry ice or at ambient in CELLSHIP®. Within 48h of recovery, cells transported in CELLSHIP® were fully adherent and were displaying typical growth morphology (A & B). Cells recovered from cryopreservation had started to adhere but had not yet reestablished typical HepG2 growth morphology (C & D)
Protocol
*Canine MSCs were transported using a cold pack as per our collaborator’s protocol. Typical temperature range that MSCs were exposed to was 5ºC – 13ºC. Simple protocol, requiring minial Cell manipulation. To date, CELLSHIP® has been validated for up to 120 h transport/storage at ambient temperatures, for a range of cell lines (Table 1) and for 72 h transport/storage of primary, canine mesenchymal stem cells (MSCs).
Recovery
Gently resuspended the cells in the cryotube and transfer the cell suspension to the appropriate volume of complete growth medium. Culture using standard conditions for 24 h to 48 h. There is no need to remove CELLSHIP® before recovery.
