300K Solutions

CellSplit Cell Dissociation Reagent

A completely Xeno-free, direct replacement to porcine or bovine-derived trypsin

CellSplit® is suitable for both serum-free and serum-containing cell culture protocols due to the inactivation by dilution. Unlike trypsin, there is no requirement for specific inhibitors and it has been developed so that you can use the same protocol, eliminating any disruption to your current procedure.

Features

CellSplit® is suitable for both serum-free and serum-containing cell culture protocols due to the inactivation by dilution. Unlike trypsin, there is no requirement for specific inhibitors, such as foetal bovine serum (FBS).

 

The active ingredient is of plant origin and manufactured to GMP grade. A cysteine protease that has broad specificity, cleaving the peptide bonds of leucine and glycine. Cysteine proteases have been extensively used for cell and tissue disassociation.

 

 

Specifications

SpecificationDetails
Available Sizes100mL, 500mL
FormulationXeno-free, plant-derived
UseCell dissociation
Active ingredientPlant-derived cysteine protease
Shelf life-------------------
Storage-------------------
ShippingAmbient temperature

Advantages of T-Store®

  • Xeno-free
  • Adaptable to your current protocol
  • Not derived from bacterial cultures
  • Plant-derived enzyme manufactured to GMP
  • No need to use inactivation agents (such as FBS),

inactivation by dilution

CellSplit® Data

CellSplit® has been comprehensively tested with a wide range of cell lines including Vero, HEK, C2, C12, L929, MRC5, LLCPK1, MDCK and CHO. It has also been tested with primary canine stem cells (FIgura 1.2.3)

Figure 1: Comparison of cell dissociation percentage rates over time between reagents on Vero cell lines. Figure shows the increased rate of the dissociation of Vero cells incubated in Cellsplit (blue) in comparison to its competitors TrypLE (green) Trypsin (red). Data shown as means (two-way ANOVA test)**** p ≤ 0.0001.   

Figure 2. Comparison of cell dissociation percentage rates over time between reagents on MRC-5 cells lines. Figure shows the increased rate of the dissociation of MRC-5 cells incubated in Cellsplit (blue) in comparison to its competitors TrypLE (green), Trypsin (red). Data shown as means (two-way ANOVA test)****p ≤ 0.0001.

Figure 3. The impact of cell harvesting procedures on releasing cytochrome c. 

The release of cytochrome c from mitochondria is a central event in apoptosis signaling that was quantifield by ELISA. Number of cells recovered by each cell dissociation reagent at 5 minutes. Some cells were trated with actinomycin-D to induce apoptotic as a positive control. (A) The release of cytochrome c protein in HEK-F cells was lower in CellSplit® (B) The level of releasing cytochome c in CellSplit® i showed low level expression in Hep-G2 cells. The data expressed as mean ± SD of technical triplicates (n=3).