T-Store® Tissue Storge and Transportation Medium insulin-free with antibiotic
Xeno-free Storage and Transport Media for mammalian tissue without insulin and with Amphotericin B and Chloramphenicol/Nanomycopulitin
T-Store®is an innovative, sterile, chemically defined and optimized media developed to provide a stable storage and shipping environment for mammalian tissue samples. This product maintains the integrity of tissue samples, thereby reducing necrosis and apoptosis and it is formulated without insulin.
T-Store® has been developed to provide a stable storage and shipping enviroment for mammalian tissue samples and cells. T-Store® utilizes a robust buffering system, allowing its use over a wide temperature range. In addition, it has been designed to be isosmotic, isotonic and isoionic with human serum and interstitial fluid, allowing the optimal storage for tissue and cells for hours or days, as required.
T-Store® allows tissue samples to retain genetic and histological profiles for up to 72 hours following removal.
Main product features:
– Sterile and Xeno-free
– Non-toxic additives
– Flexibility for highly time critical processes, homeostatic environment
– Can be used in aerobic and anaerobic conditions
– Can be used at normothermic and sub-normothermic (no frozen) temperatures
– Ambient temperature
– Antibiotic (Amphotericin B and chloramphenicol/ nanomycopulitin)
|Available Sizes||30 mL, 50mL, 125mL, 1L|
|Formulation||Xeno-free, solvent-free, insulin-free|
|Antibiotic combination||Amphotericin B and Chloramphenicol / Amphotericin B and Nanomycopulitin|
|Antibiotic concentration||Amphotericin B (5 µg/mL), Chloramphenicol (0.1 mg/mL), Nanomycopulitin (1X)|
|Use||- Storge and transport of tissue simples
- Storage of cells following dissociation /isolation of enrichment
|pH||pH 7.20 - 7.45 for temperatures between +2°C to +37°C|
|Conditions||Aerobic and Anaerobic|
|Shelf life||1 month from date of manufacture|
|Storage||+2°C to +8°C under dark conditions|
Advantages of T-Store®
Table 1. Benefits of using T-Store® for tissue simples
|Collection stage||Current Practices||T-Store® Protocols|
|Collection and inmediate holding||Biopsy delivered dry/ soaked in saline/ wrapped in gauze / formalin fixed.||Biopsies can be simply immersed in requisite volume of T-STORE® at +2ºC to 8ºC .|
|Transportation||Snap frozen (SF), Formalin fixed (FFPE) processing or sent as fresh tissue. Long transport times may degrade fresh tissues.||Tissues can be maintained in T-STORE® over ice at 0ºC to +4ºC or room temperature (RT) for up to 72 hours before processing.|
|Processing||Fresh tissues may be utilized immediately otherwise availability is limited to SF or FFPE tissue samples.||T-STORE® is suitable for use with a wide range of downstream analysis.
|Analyses||Analytical choices are limited due to processing restrictions.||T-STORE® is suitable for wide range of tissue and analytical options.|
|Outcomes||Possibility of changes in molecular profiles within tissue samples.||T-STORE® retains the morphological and molecular structure of tissue.|
Procurement of tissue samples:
T-Store® is supplied to use neat without the need for futher dilution. Following excision, tissue simples should be immediately submerged in T-Store® . This volumen should be determined by the user. It is advised to use excess volumen T-Store® to tissue weight / size ratio to ensure succesful storage.
Cell storage at ambient temperature using T-Store®
Prior to storage, cells should be cultured under recommended conditions
Each batch of T-Store® undergoes functionality testing before realese following the above protocol. Data show that Cells numbers are maintained with excellent viability and recovery, following 72H storage in T-Store® (Figure 1)
Figure1. Data collated of 14 batches´ quality control functionally test with HEK cells
To recover the cells, gently re-suspend them before transferring into an appropriate vessel, containing complete growth medium (there is no need to remove the T-Store® prior to recovery). Incubate cells under optimal conditions. Full cell recovery should be seen within 48 h.