300K Solutions

Cyto3D Live-Dead Assay Kit

Live dead cell viability analysis for 3D and 2D cell culture.


The Cyto3D Live-Dead Assay Kit is used to determine the live/dead nucleated cells by using a fast one-step staining procedure for analysis on a dual-fluorescence system. This kit is recommended for viability analysis of cells cultured in 3D, 2D coating, and on monolayer.


  • Ready-to-use
  • Fast
  • Sensitive
  • Excellent for 3D cell cultures
  • Cost-effective

Acridine orange (AO) and propidium iodide (PI), both nuclear staining (nucleic acid binding) dyes, are used in this kit. AO is permeable to both live and dead cells and stains all nucleated cells to generate green fluorescence. PI only penetrates the membranes of nucleated cells with compromised membranes and stains the dead cells to generate red fluorescence. Due to the quenching, when cells are stained with both AO and PI, all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red (the PI reduces the fluorescence intensity of the AO by fluorescence resonance energy transfer (FRET)). Non-nucleated materials such as red blood cells, platelets and debris do not fluorescence and are ignored by fluorescence microscopes.


Dual-Fluorescence Viability, using AO and PI, is the recommended viability analysis method for cell lines, primary cells, and stem cells.


FormulationPremixed acridine orange (AO) and propidium iodide (PI), nuclear staining dyes
Use Live dead cell viability analysis for 3D and 2D cell culture
Detection MethodFluorescent
Excitation/EmissionAO (494/517NM), PI (535/617nm)
Standard filtersAO (GFP), PI (Texas Red)
For use with
Fluorescence microscope, flow cytometer, microplate reader, fluorescence cell counter.
Storage2 to 8°C (Protect from light)
Shipping ConditionsShips at ambient temperature
Number of reactions500 (at 2 µL per 100 µL)


Easy setup and use