VitroGel Cell Recovery Solution
Enzyme-free cell harvesting solution to recover cells from the hydrogel in 20 min.
VitroGel Cell Recovery Solution is an enzyme-free, ready-to-use solution to harvest 2D, 3D, and organoids cultured cells efficiently and safely. The solution can recover cells from VitroGel hydrogels in 20 minutes.
VitroGel Cell Recovery Solution is room temperature stable, has a neutral pH, and works at 37°C operating temperature. The solution can maintain high cell viability during the recovery process. Harvested cells can be sub-culture in both 2D and 3D cultures.
The VitroGel Cell Recovery Solution can be used before or after the fixation and stained preparation of hydrogel specimens to ensure high-quality downstream data analysis.
- KEEP SOLUTION WARM: It is important to keep the cell recovery solution and the mixture warm at 37°C during the whole process. The warm temperature is essential to accelerate molecular exchanges to release the ionic molecules from the solid hydrogel, which can transform into a soft hydrogel.
- APPLY MECHANICAL FORCE: The mechanical force such as rocking or shaking the centrifuge tube or using a serological pipette to mix the hydrogel with the cell recovery solution helps to transform the hydrogel into the liquid state.
- DILUTION: Adding the cell recovery solution at a volume of 10X or higher than the hydrogel maintains the dissolved hydrogel in a liquid state.
- CENTRIFUGE AT ROOM TEMPERATURE
|Use||Harvest cells from VitroGel hydrogel while maintaining high cell viability.
Use before or after sample fixation and stained preparation for imaging or downstream data analysis.
|Processing Time||15-20 min|
|Downstream||Recovered cells can be sub-culture in both 2D and 3D culture.|
|Storage||Ambient Temperature (15-30°C)|
|Stability||60 months from date of manufacture.|
NEW IMPROVED VERSION
VitroGel Organoid Recovery Solution
Non-enzymatic cell harvesting solution to recover intact organoids/cells from hydrogel or an animal-based ECM within 15 minutes.