300K Solutions

VitroGel HEK293

Ready-to-use, xeno-free (animal origin-free) hydrogel system for 3D culture of HEK293 cells.

 

VitroGel HEK293 is a xeno-free (animal origin-free) functional hydrogel system developed to support three-dimensional (3D) cultures of human embryonic kidney 293 (HEK293) cells.

Features

  • Simple 5-minute protocol.
  • High-quality 3D spheroids.
  • Easily scale up with flasks and bioreactors.
  • Easy cell harvesting protocol (Enzyme-free, No trypsin).

VitroGel HEK293 is a unique system to easily support the rapid 3D growth of HEK293 cells.  Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D static suspension cultures. Both our standard 3D cell culture protocol and the 3D static suspension culture protocols can be used for rapid cell expansion. The high-quality 3D spheroids enhance the cell performance for protein expression and downstream applications. HEK293 cells cultured in VitroGel HEK293 can easily be scale-up for large-scale bioreactors or spin flasks. By using the VitroGel Cell Recovery Solution, the cell harvesting after 3D culture is simple and effective.

Specifications

SpecificationDetails
FormulationXeno-free, functional hydrogel
Use3D cell culture, 2D hydrogel coating, hydrogel-cell bead formation.
OperationReady-to-use at room temperature.
BiocompatibilityBiocompatible, safe for animal studies
InjectionInjectable hydrogel for in vivo studies and lab automation.
pHNeutral
StorageStore at 2-8°C. Ships at ambient temperature
Sizes10mL and 2mL
Number of Uses- (10 mL): 300 uses at 50 µL per well
- (2mL): 60 uses at 50 µL per well
Cell HarvestingVitroGel Organoid Recovery Solution
5-15 min cell recovery

Recommended Product

VitroGel Organoid Recovery Solution

Recover cells from the hydrogel within 15 minutes with high cell viability. Non-enzymatic system.

Methodology

“Just add cells” 5 min protocol 3D static suspension culture

VitroGel HEK293 is ready-to-use. Just mix with your cells. There is no cross-linking agent or the need to adjust the hydrogel concentration.

“Just add cells” 20 min 3D cell culture process

VitroGel HEK293 is ready-to-use. Just mix with your cells. There is no cross-linking agent or the need to adjust the hydrogel concentration.