300K Solutions

VitroGel ORGANOID

Ready-to-use, xeno-free (animal origin-free) hydrogel system for organoid culture.

 

VitroGel ORGANOID (1-4) are xeno-free (animal origin-free) hydrogels that support the growth of patient-derived organoids or organoids developed from pluripotent stem cells (PSCs), co-culture, and PDX model.

Features

  • Xeno-free hydrogel for organoid formation.
  • Supports a wide range of organoids from patient-derived samples, stem cells, tissues, co-culture and PDX resources.
  • Operation protocol at room temperature, automation-friendly.
  • Easy 20-minute cell harvesting (enzyme-free).

VitroGel ORGANOID hydrogels are ready to use at room temperature and have a neutral pH, transparent, permeable, and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. VitroGel ORGANOID hydrogels are good for both 3D cell culture and 2D hydrogel coating applications.

The VitroGel ORGANOID hydrogels can work in conjunction with VitroGel STEM (a xeno-free hydrogel for 3D static suspension cultures and scale-up of human pluripotent stem cells) by transferring stem cell spheroids cultured in VitroGel STEM to VitroGel ORGANOID hydrogels for organoid differentiation. See Xeno-free 3D Organoid Workflow B Key growth factors and molecules can be directly mixed with the hydrogel matrix or by adding on the top of the hydrogel. Organoids cultured in this system can be easily harvested out with our VitroGel Cell Recovery Solution.  VitroGel ORGANOID hydrogels provide a well-defined 3D microenvironment for the future of personalized medicine.

Which Organoid Hydrogel to choose

We still strongly suggest starting with the VitroGel ORGANOID Discovery Kit to make a quick screening to find the hydrogel version that is optimal for the organoid conditions.
 
The kit includes all four types of organoid hydrogels:
VitroGel ORGANOID-1, VitroGel ORGANOID-2, VitroGel ORGANOID-3, VitroGel ORGANOID-4)
Each of the four types has various bio-functional ligands, mechanical strengths, and degradability to fulfill the needs of different organoid culture conditions.
 
Because there is a wide range of organoid cell resources (from stem cells, patient-derived tissue, co-culture, to PDX), it is hard to tell which organoid hydrogel would best perform the researcher’s experiment. Therefore, the Discovery Kit helps researchers to screen the four different formulations to determine the best organoid hydrogel version for the organoid conditions.
 
From TheWell Bioscience findings, versions 1, 2, or 3 are suitable for gastric organoids. Versions 1 or 3 are suitable for lung organoids. Versions 2 or 3 are suitable for brain organoids, and versions 3 or 4 are suitable for cancer organoids.

3D organoid cell culture process in 20 min “Just add cells”

VitroGel ORGANOID is ready-to-use. Just mix with your cells. There is no cross-linking agent or the need to adjust the hydrogel concentration.

Specifications

SpecificationDetails
FormulationXeno-free, functional hydrogel
UseOrganoid culture
OperationReady-to-use at room temperature.
BiocompatibilityBiocompatible, safe for animal studies
InjectionInjectable hydrogel for in vivo studies and lab automation
pHNeutral
StorageStore at 2-8°C. Ships at ambient temperature
SizesSingle Vial: 10mL
Discovery Kit: (2mL) each of ORGANOID-1, ORGANOID-2, ORGANOID-3, ORGANOID-4
Number of Uses- (10 mL): 600 uses at 25 µL per dome or 300 uses at 50 µL per well
- (2mL): 120 uses at 25 µL per dome or 60 uses at 50 µL per well
Cell HarvestingVitroGel Organoid Recovery Solution
5-15 min cell recovery

Recommended Product

VitroGel Organoid Recovery Solution

Recover cells from the hydrogel within 15 minutes with high cell viability. Non-enzymatic system.

Methodology

VitroGel Organoid Culture Methods

VitroGel ORGANOID system can culture organoids in variety of methods:
3D DOME  |  2D DOME  |  2D Hydrogel Coating  |  3D Cell Encapsulation  |  3D Hydrogel-Cell Droplet

 

Review all the following VitroGel ORGANOID protocol that best fits your project.

3D Dome Method

Widely used method for organoid generation.

2D Dome Method

Add cells on top of the hydrogel dome for organoid-gel-medium interactions.

2D Hydrogel Coating Method

Add cells on top of hydrogel for optimal interactions among cells, hydrogel, and medium.

3D Cell Encapsulation Method

Encapsulate cells within the hydrogel for excellent mechanical support and the cell-matrix interactions.

3D Hydrogel-Cell Droplet Method

Create a Hydrogel-Cell droplet. This is a unique culture method only to the VitroGel system for generating great cell-matrix interactions and excellent medium penetration for organoid formation and scale-up.

Workflows

Xeno-free 3D Organoid Workflow A: 
Using VitroGel ORGANOID for organoid culture and maturation

Xeno-free 3D Organoid Workflow B:
Start from iPSC spheroids for stem cell differentiation and organoid formation.
(Diagram below shows culturing human intestinal organoid from stem cell spheroids)