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VitroGel RGD High Concentration

RGD modified – tunable, xeno-free hydrogel – high concentration (3 mL kit)

 

VitroGel RGD High Concentration is a tunable, xeno-free (animal origin-free) hydrogel system modified with cell adhesive peptide RGD to promote cell attachment and cell-matrix interactions during the 3D cell culture. VitroGel RGD High Concentration comes with VitroGel Dilution Solution to adjust the final hydrogel strength from 10 to 4000 Pa. 

Features

VitroGel High Concentration hydrogels are xeno-free, tunable hydrogels for researchers wanting full control to manipulate the biophysical and biological properties of the cell culture environment. The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. The hydrogel system has a neutral pH, transparent, permeable and compatible with different imaging systems. The solution transforms into a hydrogel matrix by simply mixing with the cell culture medium. No cross-linking agent is required. Cells cultured in this system can be easily harvested with VitroGel Cell Recovery Solution. The hydrogel can also be tuned to be injectable for in vivo studies.

From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in vivo studies with the same platform system.

Mix & Match – 3D Cell Culture Your WAY!

Unique to VitroGel High Concentration hydrogels is the ability to tailor create a multi-functional hydrogel by blending different types of VitroGel. VitroGel RGD High Concentration can be “mix & matched” with other VitroGel High Concentration hydrogels such as VitroGel IKVAV, VitroGel YIGSR, VitroGel MMP, and VitroGel COL to create a customized multi-functional hydrogel. Using this flexible and powerful hydrogel system, scientists customize their 3D culture micro-environment for different applications.

Tunable Hydrogel Strength

Simply diluting the hydrogel controls the gel strength.

Specifications

SpecificationDetails
Contents– VitroGel RGD High Concentration, 3 mL
– VitroGel Dilution Solution, 50 mL
FormulationXeno-free tunable hydrogel modified with RGD peptide.
UseGood for adhesion cells or cells requiring stronger cell-matrix interactions.
Mix & MatchCan be blended with other versions of VitroGel concentrated hydrogels to create a custom multi-functional matrix.
OperationRoom temperature
Hydrogel Strength10 to 4,000 Pa of G’ depending on dilution ratio.
Dilute with VitroGel Dilution Solution (TYPE 1
 or TYPE 2) for different concentrations.
pHNeutral
ColorTransparent
Cell HarvestingVitroGel Organoid Recovery Solution
5-15 min cell recovery
InjectableInjectable hydrogel
StorageStore at 2-8°C. Ships at ambient temperature
Number of UsesDilution ratio:
1:2 = 225 uses at 50 µL per well
1:3 = 300 uses at 50 µL per well
1:5 = 450 uses at 50 µL per well

Recommended Product

VitroGel Cell Recovery Solution

Recover cells from the hydrogel in 20 minutes with high cell viability. Enzyme-free system.

Methodology

3D Cell Culture Process in 20 Minutes

VitroGel High Concentration hydrogels are easy-to-use. There is no cross-linking agent required. Work confidently at room temperature.