DNA ROOM TEMPERATURE STABILIZATION SOLUTION
Say goodbye to freezers and discover a smarter way to preserve DNA. 300K DNA Stabilization Solution delivers unbeatable efficiency and security.
Perfect Preservation. No Freezing Needed!
Room temperature storage keeps your DNA intact and ready for analysis—no freezers, no degradation risks.
Optimize your space
Store more samples in less space by keeping them at room temperature and free up valuable freezer space.
Long-Term Stability
DNA preserved with the 300K Solution lasts up to 20 years at room temperature
Versatile Applications
Your DNA stays viable for high-level analyses like WES and SNPs.
Shipping has never been this simple.
Transport samples easily safely at room temperature and avoid the risks of cold chain logistics.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality.
PRODUCTS
The purity, integrity, and functionality of DNA stabilized with the 300K DNA Room Temperature Stabilization Solution remain of high quality up to 20 years (in accelerated studies), as demonstrated by rigorous testing according to the International Society for Biological and Environmental Repositories (ISBER) proficiency standards.
DNA purity by Spectrophotometry
Purity was assessed by comparing the absorbance ratios 260/280 and 260/230, and no difference was observed between fresh DNA (recently extracted) and DNA stored up to 18 months (1,5 years) under different storage conditions: frozen at -80 ºC (gold-standard for sample storage), RT (22 ºC) and 60 ºC (equivalent to 20 years at RT). These results show that 300K stabilization solution allows long-term RT storage of DNA without losing its purity.
| Storage Time | Sample ID | A260/280 nm | A260/230 nm |
|---|---|---|---|
| T0 | Fresh DNA | 1,82 ± 0,020 | 2,09 ± 0,14 |
| 18 months | - 80 ºC | 1,83 ± 0,016 | 2,15 ± 0,15 |
| 22 ºC | 1,82 ± 0,011 | 2,14 ± 0,18 | |
| 60 ºC | 1,80 ± 0,020 | 1,97 ± 0,11 |
DNA integrity and functionality assessment by multiplex long PCR
DNA extracted and stored for 1.5 years at -80 ºC, 22 ºC, and 60 ºC (equivalent to approximately 20 years at room temperature) was subjected to multiplex long PCR, and in all storage conditions, a 17.5 kb band was distinguishable. Additionally, we extended the analysis to DNA stored for up to 4 years under the same conditions. A 17.5 kb band was still distinguishable in all samples, although with lower intensity in the accelerated condition at 60 ºC, equivalent to 53 actual years of storage at room temperature. These results demonstrate that the 300K DNA Solution allows DNA stabilization at room temperature without losing its integrity and functionality.
300K DNA Solution can be used in multiple downstream techniques. To asses its suitability for genomic studies we performed two of the most common genetic analysis:
Whole exome sequencing (WES)
WES analyzes all exons in the genome, where most disease-causing mutations occur. It is primarily used in diagnostics and personalized medicine to identify variants responsible for genetic disorders.
The total number of variants detected showed no major differences between the different storage conditions (RT, 60ºC and the “gold standard” -80 ºC). Even after 12 months at 60 ºC (which corresponds to approximately 13 years at RT), samples showed very close values in the same metrics that at day 1, almost without variation in the Precision, Recall and F1-score values.
Single nucleotide polymorphism (SNPs) arrays
SNPs are single-nucleotide variations common among individuals. They are used to study genetic predisposition to diseases and drug responses, supporting personalized medicine and pharmacogenomics.
CytoScan® 750K array showed identical profiles between fresh DNA (A) and DNA stored 1 year at -80 ºC (B), 1 year at 22 ºC (C) or 1 year at 60 ºC (D). In addition, the CN profile revealed a small gain of 522 kb in the short arm of Chromosome 11 (11p15.4), which was observed in all the samples (E) and corresponds to a codifying zone that contains CNGA4, CCKBR and CAVIN3 (OMIM genes).
DNA stabilisation using 300K technology opens up a wide range of applications in different scientific and environmental fields.
Stabilisation of soil microbiota DNA
Soil microbiota play an essential role in the ecosystem, influencing key processes such as soil fertility, nutrient cycling and plant health. Assessing the quality of microbiota DNA in these samples is essential for studies of microbial diversity and for understanding the impact of environmental factors on bacterial communities.
We evaluated the quality of bacterial DNA from soil samples after the lyophilization process using 300K Solutions technology. Aliquots were taken from the same DNA sample and were lyophilized to compare their quality with the frozen ones.
The absorbance ratios (A260/A280 and A260/A230) and DNA concentrations remained stable after lyophilization, indicating that the process does not affect DNA purity.
| Sample ID | Nucleic Acid (ng/µL) | A260/280 | A260/230 |
|---|---|---|---|
| Frozen | 188,51 | 1,88 | 0,67 |
| Lyophilized (Aliquot 1) | 173,06 | 1,85 | 0,72 |
| Lyophilized (Aliquot 2) | 170,34 | 1,88 | 0,72 |
| Lyophilized (Aliquot 3) | 169,74 | 1,90 | 0,72 |
| Lyophilized (Aliquot 4) | 171,49 | 1,88 | 0,72 |
An equivalent length was observed in both frozen and lyophilized samples, confirming no loss or fragmentation of the DNA, thus maintaining its integrity.
Check DNA resources:
- Technical Note: «4 years of proven optimal quality with the DNA Stabilization Solution from 300K Solutions»
