300K Solutions

DNA ROOM TEMPERATURE STABILIZATION SOLUTION

Get rid of freezing conditions and stabilized nucleic acids samples at room temperature with 300K DNA solution,  adding safety and making your repository more efficient maintaining the same level of sample quality for downstream applications.

METHODOLOGY

FEATURES

  • 24 precotaed vials and storage kit formats
  • Quantity from 20ug to 80ug of purified DNA
  • Final volume of 400uL
  • Allows 20 years of room temperature storage based on stability assessment under accelerated conditions

The DNA Storage Solution has been subjected to rigorous quality control to assess DNA purity, integrity and functionality after freeze-drying and room temperature storage, following the Proficiency Standards stablished by the International Society for Biological and Environmental Repositories (ISBER).

- DNA purity by Spectrophotometry

Purity was assessed by comparing the absorbance ratios 260/280 and 260/230, and no difference was observed between fresh DNA (recently extracted) and DNA stored up to 48 months (4 years) under different storage conditions: frozen at -80 ºC (gold-standard for sample storage), RT (22 ºC) and 60 ºC (equivalent to 20 years at RT). These results show that 300K stabilization solution allows long-term RT storage of DNA without losing its purity.

Storage TimeSample IDA260/280 nmA260/230 nm
T0Fresh DNA1,82 ± 0,020 2,09 ± 0,14
18 months- 80 ºC1,83 ± 0,0162,15 ± 0,15
22 ºC1,82 ± 0,011 2,14 ± 0,18
60 ºC1,80 ± 0,0201,97 ± 0,11
48 months- 80 ºC1,85±0,0091,85±0,011
22 ºC1,80±0,0041,78±0,012
60 ºC1,83±0,0071,70±0,010

- DNA integrity and functionality assessment by multiplex long PCR

A 17.5 kb band was distinguishable in DNA stored for up to 4 years at all storage conditions (-80 ºC, 22 ºC and 60 ºC)though with lower intensity in the accelerated condition at 60ºC after 4 years of storage, equivalent to 53 actual years of storage at room temperature. These results demostrate that 300K DNA Solution for room temperature storage has no negative effect on DNA functionality and integrity, emphasising the potential of our technology as an alternative to ultra-low temperature (ULT) sample storage.

APPLICATIONS

300K DNA Solution can be used in multiple downstream applications. To asses its suitability for genomic studies we performed two of the most common genetic analysis: 

Whole exome sequencing (WES)

Total number of variants detected showed no major differences between the different storage conditions (RT, 60 ºC and the “gold standard” -80 ºC) (A). In addition, all benchmarked conditions (and their combinations) showed analogous Precision, Recall and F1-score values when comparing each timepoint with -80 ºC “gold standard” storage (B). Even after 12 months at 60 ºC (which corresponds to approximately 13 years at RT), samples showed very close values in the same metrics that at day 1, almost without variation in the Precision, Recall and F1-score values.

Single nucleotide polymorphism (SNPs) arrays

CytoScan® 750K array showed identical profiles between fresh DNA (A) and DNA stored 1 year at -80 ºC (B), 1 year at  22 ºC (C) or 1 year at 60 ºC (D). In addition, the CN profile revealed a small gain of 522 kb in the short arm of Chromosome 11 (11p15.4), which was observed in all the samples (E) and corresponds to a codifying zone that contains CNGA4, CCKBR and CAVIN3 (OMIM genes).

PRODUCTS

WP: «DNA Preservation and Storage at Room Temperature»

Poster: «Novel Dry Storage Approach for DNA Preservation at Room Temperature»

Video: «Novel Dry Storage Approach for DNA Preservation at Room Temperature»

Technical note: «4 years of proven optimal quality with the DNA Stabilization Solution from 300K Solutions»