DNA ROOM TEMPERATURE STABILIZATION SOLUTION
Say goodbye to freezers and discover a smarter way to preserve DNA. 300K DNA Stabilization Solution delivers unbeatable efficiency and security.
Perfect Preservation. No Freezing Needed!
Room temperature storage keeps your DNA intact and ready for analysis—no freezers, no degradation risks.
Optimize your space
Store more samples in less space by keeping them at room temperature and free up valuable freezer space.
Long-Term Stability
DNA preserved with the 300K Solution lasts up to 5 years at room temperature
Versatile Applications
Your DNA stays viable for high-level analyses like WES and SNPs.
Shipping has never been this simple.
Transport samples easily safely at room temperature and avoid the risks of cold chain logistics.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality.
PRODUCTS
HOW IT WORKS?
The 300K Room Temperature Stabilization Solution is a technology based on the lyophilization of biological samples in a uniform and standardized process. By removing nearly all moisture, it halts degradation processes and allows samples to be maintained at room temperature while preserving their integrity after rehydration. This process requires two key elements: the Sample Stabilization System (S³), a preset freeze-drying device developed by 300K to ensure optimal and reproducible conditions, and the Sample Stabilization Kits, which includes vials pre-coated with specific excipients that protect the sample during lyophilization without interfering in downstream analyses—so no washing is needed after rehydration. This combined system ensures reliable and ready-to-use stabilization, much more than a storage tube.
The purity, integrity, and functionality of DNA stabilized with the 300K DNA Room Temperature Stabilization Solution remain of high quality, as demonstrated by rigorous testing according to the International Society for Biological and Environmental Repositories (ISBER) proficiency standards.
DNA purity by Spectrophotometry
Purity was assessed by comparing the absorbance ratios 260/280 and 260/230, and no difference was observed between fresh DNA (recently extracted) and DNA stored up to 48 months under different storage conditions: frozen at -80 ºC (gold-standard for sample storage), RT (22 ºC) and 60 ºC. These results show that 300K stabilization solution allows long-term RT storage of DNA without losing its purity.
| Storage time | Sample ID | A260/280 nm | A260/230 nm |
|---|---|---|---|
| T0 | Fresh | 1.82 ± 0.020 | 2.09 ± 0.140 |
| 18 months | - 80 ºC | 1.83 ± 0.016 | 2.15 ± 0.150 |
| 22 ºC | 1.82 ± 0.011 | 2.14 ± 0.180 | |
| 60 ºC | 1.80 ± 0.020 | 1.97 ± 0.110 | |
| 48 months | - 80 ºC | 1.85 ± 0.008 | 1.85 ± 0.110 |
| 22 ºC | 1.80 ± 0.004 | 1.78 ± 0.001 | |
| 60 ºC | 1.83 ± 0.007 | 1.70 ± 0.010 |
Figure. Measurement of DNA purity and quantity with spectrophotometry (using Nanodrop OneC)
Figure. DNA integrity and functionality assessed by multiplex long PCR.
DNA integrity and functionality assessment by multiplex long PCR
DNA extracted and stored for 18 months at -80 ºC, 22 ºC, and 60 ºC was subjected to multiplex long PCR, and in all storage conditions, a 17.5 kb band was distinguishable.
Additionally, we extended the analysis to DNA stored for up to 48 months under the same conditions. A 17.5 kb band was still distinguishable in all samples, although with lower intensity at 60 ºC.
These results demonstrate that the 300K DNA Solution allows DNA stabilization at room temperature without losing its integrity and functionality.
300K DNA Solution can be used in multiple downstream techniques. To asses its suitability for genomic studies we performed two of the most common genetic analysis:
Whole exome sequencing (WES)
WES analyzes all exons in the genome, where most disease-causing mutations occur. It is primarily used in diagnostics and personalized medicine to identify variants responsible for genetic disorders.
To assess whether DNA stabilization at room temperature with 300K technology affects downstream analysis, we compared WES variants detected in dried samples of 2 donors stored at RT (22ºC) and 60ºC to a pool of different aliquots of the same samples frozen and stored at-80 ºC (“gold standard”) for different times
Figure. Workflow for whole exome sequencing and variant calling comparison against -80 °C gold standard.
The total number of variants detected showed no major differences between the different storage conditions (RT, 60ºC and the “gold standard” -80 ºC). Even after 12 months at 60 ºC. Samples showed very close values in the same metrics that at day 1, almost without variation in the Precision, Recall and F1-score values.
Figure. Comparison of total variants detected between samples stored at -80 °C and RT.
Figure. Variants detected and performance metrics (Precision, Recall, F1 score) across storage conditions and time points.
Single nucleotide polymorphism (SNPs) arrays
SNPs are single-nucleotide variations common among individuals. They are used to study genetic predisposition to diseases and drug responses, supporting personalized medicine and pharmacogenomics.
Genome-wide analysis using high density SNP arrays provided characterization of the donor samples through the identification of copy number (CN) changes and loss of heterozygosity (LOH) for millions of SNPs. CytoScan 750K array. showed identical profiles between fresh DNA (A) and DNA stored 1 year at -80 ºC (B), 1 year at 22 ºC (C) or 1 year at 60 ºC (D). In addition, the CN profile revealed a small gain of 522 kb in the short arm of Chromosome 11 (11p15.4), which was observed in all the samples (E) and corresponds to a codifying zone that contains CNGA4, CCKBR and CAVIN3 (OMIM genes).
Figure. Workflow for Single Nucleotide Polymorphism arrays
Figure. Cytogenetic analysis using SNP arrays from DNA. Highlighted with red rectangles, a small gain in the short arm of chromosome 11 (11p15.4) was observed in all the aliquots analyzed
DNA stabilisation using 300K technology opens up a wide range of applications in different scientific and environmental fields.
Stabilization of soil microbiota DNA
Soil microbiota play an essential role in the ecosystem, influencing key processes such as soil fertility, nutrient cycling and plant health. Assessing the quality of microbiota DNA in these samples is essential for studies of microbial diversity and for understanding the impact of environmental factors on bacterial communities.
We evaluated the quality of bacterial DNA from soil samples after the lyophilization process using 300K Solutions technology. Aliquots were taken from the same DNA sample and were lyophilized to compare their quality with the frozen ones.
Figure. Workflow for stabilization of soil microbiota
The absorbance ratios (A260/A280 and A260/A230) and DNA concentrations remained stable after lyophilization, indicating that the process does not affect DNA purity.
| Sample ID | Nucleic Acid (ng/µL) | A260/280 | A260/230 |
|---|---|---|---|
| Frozen | 188.51 | 1.88 | 0.67 |
| Lyophilized (Aliquot 1) | 173.06 | 1.85 | 0.72 |
| Lyophilized (Aliquot 2) | 170.34 | 1.88 | 0.72 |
| Lyophilized (Aliquot 3) | 169.74 | 1.90 | 0.72 |
| Lyophilized (Aliquot 4) | 171.49 | 1.88 | 0.72 |
Table. DNA concentration and purity (A260/280 and A260/230 ratios) in frozen and lyophilized samples.
An equivalent length was observed in both frozen and lyophilized samples, confirming no loss or fragmentation of the DNA, thus maintaining its integrity.
Figure. DNA integrity comparison between frozen and lyophilized samples by gel electrophoresis.
Quality control and standardisation in biobanks
DNA samples freeze dried with 300k Solutions technology have been evaluated in a comparison study coordinated by the Carlos III National DNA Bank, in which 14 Spanish biobanks participated.
The aim of the study was to analyse and compare DNA samples of different quality that have been freeze-dried and stored at room temperature with fresh DNA samples in order to evaluate the possible implementation of freeze-drying as a method of DNA processing and storage in the normal workflow of a biobank.
Figure. Workflow for quality control and standardization of DNA samples in biobanks using 300K stabilization solution.
The quality control results obtained on fresh and freeze-dried samples for each DNA quality showed that, in terms of purity, there are no differences regardless of the laboratory, instrumentation or operator involved in the process. However, a slight decrease in concentration was observed in the freeze-dried samples compared to the fresh samples, which in no case invalidates their subsequent use.
Figure. Correlation of DNA concentration and purity ratios (A260/280 and A260/230) between freeze-dried and fresh samples for different sample quality categories.
Figure. Paired comparison of DNA concentration and purity ratios for different sample quality categories.
In view of these results, we can conclude that freeze-drying is a real alternative for the processing, transport and storage of DNA samples, opening the door to its implementation in the workflow of biobanks, saving freezing space, operating costs and gaining security by not depending on cold chains.
