FLOW CYTOMETRY THRESHOLD DETERMINATION
The SPHERO™ Blank Calibration Particles are designed for the determination of the flow cytometer’s fluorescence threshold. It is important to determine if the autofluorescence level of a sample is above the fluorescence threshold of the instrument. To measure the fluorescence threshold of the instrument run the Blank Calibration Particles at the same instrument settings and determine if they are less fluorescent than the sample. The fluorescence threshold of the instrument can be quantitatively determined by calibrating the instrument using the same instrument settings with calibrated standards such as the Rainbow or Ultra Rainbow Calibration Particles.
SPHERO Blank Calibration Particles
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
|---|---|---|---|---|
| BCP-10-5 | Blank Calibration Particles | 107/mL | 1.0-1.4 µm | 5 mL |
| BCP-20-5 | Blank Calibration Particles | 107/mL | 1.8-2.2 µm | 5 mL |
| BCP-30-5 | Blank Calibration Particles | 107/mL | 3.2 (+/-0.1 µm) | 5 mL |
| BCP-32-5 | Blank Calibration Particles | 107/mL | 3.0-3.4 µm | 5 mL |
| BCP-35-5 | Blank Calibration Particles | 107/mL | 3.5-4.0 µm | 5 mL |
| BCP-50-5 | Blank Calibration Particles | 107/mL | 5.0-5.9 µm | 5 mL |
| BCP-60-5 | Blank Calibration Particles | 107/mL | 6.0-6.4 µm | 5 mL |
| BCP-70-5 | Blank Calibration Particles | 107/mL | 6.5-8.0 µm | 5 mL |
| BCP-100-5 | Blank Calibration Particles | 5x106/mL | 8.0-12.9 µm | 5 mL |
| BCP-150-5 | Blank Calibration Particles | 5x106/mL | 13.0-17.9 µm | 5 mL |
| BCP-200-5 | Blank Calibration Particles | 0.5x106/mL | 18.0-24.9 µm | 5 mL |
| BCP-300-5 | Blank Calibration Particles | 0.5x106/mL | 25.0-35.0 µm | 5 mL |
