PERIPHERAL BLOOD ROOM TEMPERATURE STABILIZATION SOLUTION
300K Peripheral Blood Stabilization Solution delivers precision storage at room temperature, eliminating the complexities of freezing and ensuring reliable results for a wide range of clinical applications.
Perfect Preservation. No Freezing Needed!
Room temperature storage keeps your peripheral blood intact and ready for analysis—no freezers, no degradation risks.
Optimize your space
Store more samples in less space by keeping them at room temperature and free up valuable freezer space.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality.
Versatile Applications
Your DNA stays viable for high-level analyses like WES and SNPs.
Shipping has never been this simple.
Transport samples easily safely at room temperature and avoid the risks of cold chain logistics.
PRODUCTS
HOW IT WORKS?
The 300K Room Temperature Stabilization Solution is a technology based on the lyophilization of biological samples in a uniform and standardized process. By removing nearly all moisture, it halts degradation processes and allows samples to be maintained at room temperature while preserving their integrity after rehydration. This process requires two key elements: the Sample Stabilization System (S³), a preset freeze-drying device developed by 300K to ensure optimal and reproducible conditions, and the Sample Stabilization Kits, which includes vials pre-coated with specific excipients that protect the sample during lyophilization without interfering in downstream analyses—so no washing is needed after rehydration. This combined system ensures reliable and ready-to-use stabilization, much more than a storage tube.
DNA functionality and integrity in blood stabilized samples after 18 months of RT storage
QC of DNA extracted from blood samples freeze-dried with 300K Peripheral Blood Stabilization kit showed good purity and integrity when assessed by spectrophotometry and agarose gel electrophoresis, respectively.
| Sample name | Concentration (ng/µL) | A260/280 | A260/230 |
|---|---|---|---|
| Donor 1 | 118.073 | 1.848 | 2.071 |
| Donor 2 | 169.181 | 1.823 | 2.338 |
| Donor 3 | 159.214 | 2.248 | 1.509 |
| Donor 4 | 124.157 | 1.836 | 2.198 |
| Donor 5 | 119.314 | 1.867 | 2.151 |
Table. DNA concentration and purity ratios (A260/280 and A260/230) for blood samples stored at room temperature for 18 months.
Moreover, we additionally performed a multiplex long PCR, and all samples displayed a 17,5 kb band, confirming that our technology has no negative effect on DNA functionality after 18 months of RT storage.
Figure. DNA integrity and functionality assessed by multiplex long PCR after 18 months of RT storage.
DNA extraction for advanced genetics applications
Quality control of DNA extracted from samples freeze-dried with 300K Peripheral Blood Solution showed good purity and integrity when assessed by spectrophotometry and agarose gel electrophoresis, respectively (data not shown). Moreover, we additionally performed a multiplex long PCR, and all samples displayed a 17,5 kb band, confirming that our technology has no negative effect on DNA functionality.
Figure. Workflow for DNA extraction and functionality assessment using multiplex long PCR in freeze-dried blood samples.
Figure. DNA integrity and functionality confirmed by multiplex long PCR for fresh, frozen, and freeze-dried samples.
Flow cytometry - Preliminary Proof-of-Concept Results
The first results from this proof-of-concept study indicate that whole blood samples stabilized with 300K technology remain compatible with flow cytometry analysis after room-temperature storage. Following rehydration, immune cell subpopulation frequencies closely resemble those obtained from fresh samples, suggesting promising preservation of cellular composition and surface markers.
Figure. Workflow for flow cytometry analysis using in freeze-dried blood samples.
Cell recovery
Absolute cell counts were analyzed in fresh, dried, and frozen samples. Compared to fresh samples, dried samples showed a 25.63% decrease, while frozen samples exhibited a more pronounced reduction of 44.56%.
Figure A. Cell recovery after storage across donors (Fresh vs. Dried vs. Frozen).
Relative distribution of cell subpopulations
To evaluate the stability of cell subpopulations, lymphoid and myeloid cells were analyzed separately. The results indicated that dried samples preserved frequencies similar to those observed in fresh samples across the major cell types. In contrast, frozen samples exhibited a selective reduction in myeloid cells, particularly neutrophils, which led to an imbalance in the overall subpopulation distribution.
Figures B-D. Comparison of lymphocytes, monocytes, and neutrophils across donors (Fresh vs. Dried vs. Frozen).
Further analysis demonstrated that dried samples maintained an excellent correlation with fresh samples, with an R² value close to 0.99, confirming their reliability for preserving cellular composition. Conversely, frozen samples showed a lower correlation (R² = 0.56), reflecting greater variability and reduced consistency in subpopulation frequencies.
Figures E-F. Correlation plots of cell subpopulation frequencies between fresh and stored samples.
