PLASMA/SERUM ROOM TEMPERATURE STABILIZATION SOLUTION
Transform the way you work with plasma and serum. With the 300K Plasma and Serum Solution, you gain efficiency, reliability, and peace of mind maintaining the integrity of your plasma and serum for all your future applications!
Perfect Preservation. No Freezing Needed!
Room temperature stabilization keeps your plasma and serum intact and ready for analysis—no freezers, no degradation risks.
Optimize your space
Store more samples in less space by keeping them at room temperature and free up valuable freezer space.
Versatile Applications
Your plasma and serum stays viable for high-level analyses.
Shipping has never been this simple.
Transport samples easily safely at room temperature and avoid the risks of cold chain logistics.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality.
PRODUCTS
Protein profiles by SDS-PAGE
To assess plasma quality after the freeze-drying process, we performed SDS-PAGE to compare protein profiles between freeze-dried and frozen plasma samples.
As expected, the same profile was observed in both type of samples, confirming that the technology developed by 300K Solutions does not alter plasma protein composition.
The Plasma and Serum Solution developed by 300K Solutions has been subjected to rigorous quality control after freeze-drying and room temperature storage to assess plasma composition and properties.
Proteomics study
Aliquots from plasma samples obtained from peripheral blood of 4 different donors were frozen and stored at -80ºC while other aliquots were dried and stabilized at RT using 300K Plasma and Serum Room Temperature Stabilization Solution.
After 6 months of storage, one aliquot of each donor and condition was thawed (-80 ºC storage) or rehydrated (RT storage). These samples were then subjected to proteomic analysis with liquid chromatography/mass spectrometry (LC/MS) by oloBion SL (Barcelona, Spain)
The proteomic analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 285 proteins distributed in 259 families.
When compared, 32 proteins showed significant differences between freeze-dried and frozen samples (pWelch <0,05). These 32 proteins showed higher levels in freeze-dried samples.
Each dot represents a detected protein in the plasma samples. The X-axis shows the abundance in samples frozen at -80 °C, while the Y-axis shows the abundance in samples lyophilized and stored at room temperature. Dots close to the diagonal line indicate high similarity between both storage conditions.
list of the best preserved proteins during freeze-drying
| Apolipoprotein L1 | Complement C1q subcomponent subunit C |
| Inter-alpha-trypsin inhibitor heavy chain H2 | Complement C1q subcomponent subunit A |
| Clusterin | Apolipoprotein C-II |
| Immunoglobulin lambda constant 3 | Apolipoprotein A-II |
| N-acetylmuramoyl-L-alanine amidase | Immunoglobulin heavy constant gamma 2 |
| Fetuin-B | Immunoglobulin heavy constant gamma 1 |
| Trypsin | Immunoglobulin kappa constant |
| Thyroxine-binding globulin | Immunoglobulin heavy variable 3-33 |
| Immunoglobulin lambda constant 7 | Beta-2-glycoprotein 1 |
| Apolipoprotein D | Zinc-alpha-2-glycoprotein |
| Apolipoprotein B-100 | Immunoglobulin lambda-like polypeptide 5 |
| Coagulation factor V | Immunoglobulin kappa variable 1-5 |
| Plasma kallikrein | Complement factor H-related protein 1 |
| Serotransferrin | Extracellular matrix protein 1 |
| Transthyretin | Plasminogen |
| Complement C1q subcomponent subunit C | Alpha-2-antiplasmin |
| Complement C1q subcomponent subunit A | Serum paraoxonase/arylesterase 1 |
Lipidomics study
Aliquots from plasma samples obtained from peripheral blood of 4 different donors were frozen and stored at -80ºC while other aliquots were dried and stabilized at RT using 300K Plasma and Serum Room Temperature Stabilization Solution.
After 6 months of storage, one aliquot of each donor and condition was thawed (-80 ºC storage) or rehydrated (RT storage).. These samples were then subjected to lipidomic analysis with liquid chromatography/mass spectrometry (LC/MS) by oloBion SL (Barcelona, Spain)
The lipidomic analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 548 lipids distributed in 6 categories, 23 main classes and 51 subclasses.
When compared, all lipid subclasses showed no significant differences between freeze-dried and frozen samples (pWelch <0,05). Moreover, most of the lipids identified and quantified showed no significant differences between freeze-dried and frozen samples, with the exception of 11 lipids (pWelch <0,05).
Each dot represents an individual lipid. The plot compares its abundance in frozen versus lyophilized plasma samples. Dots aligned with the diagonal indicate no relevant changes between the two storage methods, signaling high stability of the lipid profile.
Metabolomics study
The metabolomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 203 metabolites distributed in 10 superclasses and 34 classes.
When compared, all metabolic superclasses showed no significant differences between freeze-dried and frozen samples (pWelch <0,05). Moreover, most of the metabolites identified and quantified showed no significant differences between feeze-dried and frozen samples, with the exception of 27 metabolites (pWelch <0,05).
This scatter plot compares the relative abundance of each metabolite under the two storage methods. Each dot corresponds to a specific metabolite. The closer the dots are to the diagonal, the consistent the more results between frozen and room temperature-stabilized samples.
Extraction of circulating free DNA for liquid biopsy
Circulating free DNA (cfDNA) is well preserved in plasma samples stabilized with the technology of 300K Solutions.
The quantity and quality of cfDNA extracted frm both lyophilized samples stored at room temperature and samples stored at -80ºC is comparable.
Sensibility of Inmunoglobin detection levels by turbidimetry (Optilite, The Binding Site)
Same levels of monoclonal protein were detected after freeze-drying using 300K Serum and Plasma Solution when compared with the fresh serum sample.
Sensibility of Inmunoglobin detection levels by mass-spectrometry (QIP-MS, The Binding Site)
Same levels of monoclonal protein were detected after freeze-drying using 300K Serum and Plasma Solution when compared with the fresh serum sample.
