300K Solutions

PLASMA AND SERUM ROOM TEMPERATURE STABILIZATION SOLUTION

Get rid of freezing conditions and maintain Serum and Plasma samples at room temperature with 300K Serum and Plasma  Solution adding safety and making your repository more efficient maintaining the same level of sample quality for downstream applications.

METHODOLOGY

FEATURES

  • 24 precotaed vials with stoppers
  • Final volume of 500uL-1000uL

QUALITY CONTROL


Coming soon…

APPLICATIONS

 

The Plasma and Serum Solution developed by 300K Solutions has been subjected to rigorous quality control after freeze-drying and room temperature storage to assess plasma composition and properties.

       – Sensibility of Inmunoglobin detection levels by turbidimetry (Optilite, The Binding Site)

 

Turbidimetry assays to measure low levels or high levels of inmunoglobulins showed a similar recovery between fresh serum samples and freeze-dried using 300K Solutions Serum and Plasma Solution. On the other hand, when compared with frozen serum samples, the recovery showed a higher variability under this storage condition.

       

       – Sensibility of Inmunoglobin detection levels by mass-spectrometry (QIP-MS, The Binding Site)

 

Same levels of monoclonal protein were detected after freeze-drying using 300K Serum and Plasma Solution when compared with the fresh serum sample.

     

       – Lipidomics study

 

The lipidomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 833 lipids distributed in 7 categories, 21 main classes and 56 subclasses. 

When compared, all lipids class except the GDCAE class showed no significant differences between freeze-dried and frozen samples (pWelch <0,05). Moreover, most of the lipids identified and quantified showed no significant differences between freeze-dried and frozen samples, with the exception of 19 lipids (pWelch <0.05).

       – Metabolomics study

 

The metabolomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 146 metabolites distributed in 8 superclasses and 27 classes.

 

This analysis was made without considering organic oxygen compounds. When compared, all  metabolic superclasses except the homogeneous not metal compounds superclass showed no significant differences between freeze-dried and frozen samples (pWelch <0,05). Moreover, most of the metabolites identified and quantified showed no significant differences between freeze-dried and frozen samples, with the exception of 39 metabolites (pWelch <0.05).

       – Proteomics study

 

The proteomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 256 proteins distributed in 243 families and involved in 187 biological processes and 23 pathways 

When compared, all proteins showed no significant differences between freeze-dried and frozen samples (pWelch <0,05). 

Moreover, chromatograms from all donors were similar in both freeze-dried and frozen samples. [Only results of donor 4 are shown].

       – Extraction of circulating free DNA for liquid biopsy

 

Circulating free DNA (cfDNA) is well preserved in plasma samples stabilized with the technology of 300K Solutions.

The quantity and quality of cfDNA extracted frm both lyophilized samples stored at room temperature and samples stored at -80ºC is comparable.

PRODUCTS


RESOURCES

Technical note: «cfDNA extraction from freeze-dried plasma samples using 300K Solutions Plasma and Serum Stabilization Kit»

Poster: «New approachs for Room Temperature Stabilization and Storage of high Quality Plasma Samples»