PLASMA AND SERUM ROOM TEMPERATURE STABILIZATION SOLUTION
Get rid of freezing conditions and maintain Serum and Plasma samples at room temperature with 300K Serum and Plasma Solution adding safety and making your repository more efficient maintaining the same level of sample quality for downstream applications.
PRODUCTS
METHODOLOGY
PERFORMANCE
Protein profiles by SDS-PAGE
To assess plasma quality after the freeze-drying process, we performed SDS-PAGE to compare protein profiles between freeze-dried and frozen plasma samples.
As expected, the same profile was observed in both type of samples, confirming that the technology developed by 300K Solutions does not alter plasma protein composition.
APPLICATIONS
The Plasma and Serum Solution developed by 300K Solutions has been subjected to rigorous quality control after freeze-drying and room temperature storage to assess plasma composition and properties.
Proteomics study
The proteomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 256 proteins distributed in 243 families and involved in 187 biological processes and 23 pathways
When compared, all proteins showed no significant differences between freeze-dried and frozen samples (pWelch <0,05).
Moreover, chromatograms from all donors were similar in both freeze-dried and frozen samples. [Only results of donor 4 are shown].
Lipidomics study
The lipidomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 833 lipids distributed in 7 categories, 21 main classes and 56 subclasses.
When compared, all lipids class except the GDCAE class showed no significant differences between freeze-dried and frozen samples (pWelch <0,05). Moreover, most of the lipids identified and quantified showed no significant differences between freeze-dried and frozen samples, with the exception of 19 lipids (pWelch <0.05).
Metabolomics study
The metabolomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 146 metabolites distributed in 8 superclasses and 27 classes.
This analysis was made without considering organic oxygen compounds. When compared, all metabolic superclasses except the homogeneous not metal compounds superclass showed no significant differences between freeze-dried and frozen samples (pWelch <0,05). Moreover, most of the metabolites identified and quantified showed no significant differences between freeze-dried and frozen samples, with the exception of 39 metabolites (pWelch <0.05).
Extraction of circulating free DNA for liquid biopsy
Circulating free DNA (cfDNA) is well preserved in plasma samples stabilized with the technology of 300K Solutions.
The quantity and quality of cfDNA extracted frm both lyophilized samples stored at room temperature and samples stored at -80ºC is comparable.
Sensibility of Inmunoglobin detection levels by turbidimetry (Optilite, The Binding Site)
Same levels of monoclonal protein were detected after freeze-drying using 300K Serum and Plasma Solution when compared with the fresh serum sample.
Sensibility of Inmunoglobin detection levels by mass-spectrometry (QIP-MS, The Binding Site)
Same levels of monoclonal protein were detected after freeze-drying using 300K Serum and Plasma Solution when compared with the fresh serum sample.
RESOURCES
Technical note: «cfDNA extraction from freeze-dried plasma samples using 300K Solutions Plasma and Serum Stabilization Kit»
Poster: «New approachs for Room Temperature Stabilization and Storage of high Quality Plasma Samples»
