300K Solutions

PLASMA AND SERUM ROOM TEMPERATURE STABILIZATION SOLUTION

Get rid of freezing conditions and maintain Serum and Plasma samples at room temperature with 300K Serum and Plasma  Solution adding safety and making your repository more efficient maintaining the same level of sample quality for downstream applications.

PRODUCTS

METHODOLOGY

QUALITY CONTROL

Protein profiles by SDS-PAGE

To assess plasma quality after the freeze-drying process, we performed SDS-PAGE to compare protein profiles between freeze-dried and frozen plasma samples.

As expected, the same profile was observed in both type of samples, confirming that the technology developed by 300K Solutions does not alter plasma protein composition. 

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APPLICATIONS

 

The Plasma and Serum Solution developed by 300K Solutions has been subjected to rigorous quality control after freeze-drying and room temperature storage to assess plasma composition and properties.

Proteomics study

The proteomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 256 proteins distributed in 243 families and involved in 187 biological processes and 23 pathways 

When compared, all proteins showed no significant differences between freeze-dried and frozen samples (pWelch <0,05). 

Moreover, chromatograms from all donors were similar in both freeze-dried and frozen samples. [Only results of donor 4 are shown].

The lipidomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 833 lipids distributed in 7 categories, 21 main classes and 56 subclasses. 

When compared, all lipids class except the GDCAE class showed no significant differences between freeze-dried and frozen samples (pWelch <0,05). Moreover, most of the lipids identified and quantified showed no significant differences between freeze-dried and frozen samples, with the exception of 19 lipids (pWelch <0.05).

The metabolomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 146 metabolites distributed in 8 superclasses and 27 classes.

This analysis was made without considering organic oxygen compounds. When compared, all  metabolic superclasses except the homogeneous not metal compounds superclass showed no significant differences between freeze-dried and frozen samples (pWelch <0,05). Moreover, most of the metabolites identified and quantified showed no significant differences between freeze-dried and frozen samples, with the exception of 39 metabolites (pWelch <0.05).

Circulating free DNA (cfDNA) is well preserved in plasma samples stabilized with the technology of 300K Solutions.

The quantity and quality of cfDNA extracted frm both lyophilized samples stored at room temperature and samples stored at -80ºC is comparable.

Same levels of monoclonal protein were detected after freeze-drying using 300K Serum and Plasma Solution when compared with the fresh serum sample.

Same levels of monoclonal protein were detected after freeze-drying using 300K Serum and Plasma Solution when compared with the fresh serum sample.


RESOURCES

Technical note: «cfDNA extraction from freeze-dried plasma samples using 300K Solutions Plasma and Serum Stabilization Kit»

Poster: «New approachs for Room Temperature Stabilization and Storage of high Quality Plasma Samples»