PLASMA/SERUM ROOM TEMPERATURE STABILIZATION SOLUTION
Transform the way you work with plasma and serum. With the 300K Plasma and Serum Solution, you gain efficiency, reliability, and peace of mind maintaining the integrity of your plasma and serum for all your future applications!
Perfect Preservation. No Freezing Needed!
Room temperature stabilization keeps your plasma and serum intact and ready for analysis—no freezers, no degradation risks.
Optimize your space
Store more samples in less space by keeping them at room temperature and free up valuable freezer space.
Versatile Applications
Your plasma and serum stays viable for high-level analyses.
Shipping has never been this simple.
Transport samples easily safely at room temperature and avoid the risks of cold chain logistics.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality.
PRODUCTS
HOW IT WORKS?
The 300K Room Temperature Stabilization Solution is a technology based on the lyophilization of biological samples in a uniform and standardized process. By removing nearly all moisture, it halts degradation processes and allows samples to be maintained at room temperature while preserving their integrity after rehydration. This process requires two key elements: the Sample Stabilization System (S³), a preset freeze-drying device developed by 300K to ensure optimal and reproducible conditions, and the Sample Stabilization Kits, which includes vials pre-coated with specific excipients that protect the sample during lyophilization without interfering in downstream analyses—so no washing is needed after rehydration. This combined system ensures reliable and ready-to-use stabilization, much more than a storage tube.
Protein profiles by SDS-PAGE
To assess plasma quality after the freeze-drying process, we performed SDS-PAGE to compare protein profiles between freeze-dried and frozen plasma samples.
As expected, after 12 months stored at room temperature, the same profile was observed in both type of samples, confirming that the technology developed by 300K Solutions does not alter plasma protein composition.
Figure. Protein profile comparison between freeze-dried and frozen plasma samples by SDS-PAGE after 12 months of storage.
The Plasma and Serum Solution developed by 300K Solutions has been subjected to rigorous quality control after freeze-drying and room temperature storage to assess plasma composition and properties.
Proteomics study
Aliquots from plasma samples obtained from peripheral blood of 4 different donors were frozen and stored at -80ºC while other aliquots were dried and stabilized at RT using 300K Plasma and Serum Room Temperature Stabilization Solution.
After 12 months of storage, one aliquot of each donor and condition was thawed (-80 ºC storage) or rehydrated (RT storage). These samples were then subjected to proteomic analysis with liquid chromatography/mass spectrometry (LC/MS) by oloBion SL (Barcelona, Spain).
Figure. Workflow for proteomic analysis of plasma samples using LC/MS after 12 months of storage.
The proteomic analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 258 proteins.
Figure. Profile of 258 proteins detected in freeze-dried plasma samples.
A Pearson correlation coefficient was calculated to evaluate the relationship between the proteins detected in frozen plasma samples and the ones detected in freeze-dried samples. There was a significant and positive (strong) relationship between the proteins present in both types of samples, r([258])=[0.994], p=[<0.01].
Figure. Pearson correlation values between freeze-dried and frozen plasma samples showed no differences between both storage methods.
Lipidomics study
Aliquots from plasma samples obtained from peripheral blood of 4 different donors were frozen and stored at -80ºC while other aliquots were dried and stabilized at RT using 300K Plasma and Serum Room Temperature Stabilization Solution.
After 12 months of storage, one aliquot of each donor and condition was thawed (-80 ºC storage) or rehydrated (RT storage). These samples were then subjected to lipidomic analysis with liquid chromatography/mass spectrometry (LC/MS) by oloBion SL (Barcelona, Spain).
Figure. Workflow for lipidomic analysis of plasma samples using LC/MS after 12 months of storage.
The lipidomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 938 lipids distributed in 7 categories.
FIgure. Profile of 938 lipids detected in freeze-dried plasma samples.
A Pearson correlation coefficient was calculated to evaluate the relationship between the lipids detected in frozen plasma samples and the ones detected in freeze-dried samples. There was a significant and positive (strong) relationship between the lipids present in both types of samples, r([938]) = [0.993], p = [< 0.01]
Figure. Pearson correlation values between freeze-dried and frozen plasma samples showed no differences between both storage methods.
Metabolomics study
Aliquots from plasma samples obtained from peripheral blood of 4 different donors were frozen and stored at -80ºC while other aliquots were dried and stabilized at RT using 300K Plasma and Serum Room Temperature Stabilization Solution.
After 12 months of storage, one aliquot of each donor and condition was thawed (-80 ºC storage) or rehydrated (RT storage). These samples were then subjected to metabolomic analysis with liquid chromatography/mass spectrometry (LC/MS) by oloBion SL (Barcelona, Spain)
Figure. Workflow for metabolomic analysis of plasma samples using LC/MS after 12 months of storage.
The metabolomics analysis in freeze-dried and frozen plasma samples from all 4 donors identified a total of 254 metabolites distributed in 27 superclasses.
Figure. Profile of 254 metabolites detected in freeze-dried plasma samples.
A Pearson correlation coefficient was calculated to evaluate the relationship between the metabolites detected in frozen plasma samples and the ones detected in freeze-dried samples. There was a significant and positive (strong) relationship between the metabolites present in both types of samples r([254]) = [0.998], p = [< 0.01]
Figure. Pearson correlation values between freeze-dried and frozen plasma samples showed no differences between both storage methods.
Extraction of circulating free DNA for liquid biopsy
Circulating free DNA (cfDNA) is well preserved in plasma samples stabilized with the technology of 300K Solutions.
The quantity and quality of cfDNA extracted from both lyophilized samples stored at room temperature and samples stored at -80ºC is comparable.
Figure. Workflow for cfDNA extraction and concentration measurement from plasma samples.
| Sample | [cfDNA] -80 ºC | [cfDNA] RT storage |
|---|---|---|
| 1 | 0.282 ng/µL | 0.314 ng/µL |
| 2 | 0.126 ng/µL | 0.170 ng/µL |
| 3 | 0.108 ng/µL | 0.228 ng/µL |
| 4 | 0.270 ng/µL | 0.142 ng/µL |
Table. cfDNA concentration in plasma samples stored at -80 °C and at room temperature.
Figure. cfDNA fragment size distribution for samples stored at -80 °C and at room temperature.
Sensibility of Inmunoglobin detection levels by turbidimetry (Optilite, The Binding Site)
Same levels of monoclonal protein were detected after freeze-drying using 300K Serum and Plasma Solution when compared with the fresh serum sample.
Figure. Comparison of monoclonal protein levels in fresh, frozen, and freeze-dried serum samples at low and high Ig concentrations.
Sensibility of Inmunoglobin detection levels by mass-spectrometry (QIP-MS, The Binding Site)
Same levels of monoclonal protein were detected after freeze-drying using 300K Serum and Plasma Solution when compared with the fresh serum sample.
Figure. Comparison of monoclonal protein profiles between fresh and freeze-dried serum samples using LC/MS analysis.
Scientific Resources Developed by 300K Solutions
External Scientific References
Independent studies and collaborations validating 300K Solutions technology in plasma sample stabilization.
- Poster: «Freeze-drying as a Sustainable and Safe Strategy for Storing Biological Samples in Biobanks: Pilot Study» Presented by the Biobank of Hospital Universitario Puerta de Hierro at the III Jornadas de las Plataformas ISCIII 2025. (See English version)
- Poster: «Comparison of Cytokine concentrations in Ultrafrozen vs lyophilised plasma form MDS patients by Luminex» Presented by IBSAL at the 18th International Congress on Myelodysplastic Syndromes Conference.
- Poster: «Comparison of Cytokine concentration in frozen plasma compared to lyophilised plasma from patients with MDS using the Luminex technique» Presented by IBSAL at the 36th AETEL National Congress 2025 ‘Mass Sequencing’ conference. (See English version)
