RNA ROOM TEMPERATURE STABILIZATION SOLUTION
Tired of worrying about your RNA degrading? Now you can stop stressing about unstable samples, 300K RNA Stabilization Solution keeps your RNA secure at room temperature, making storage and transport easier than ever!
Add security
RNA is fragile, but with the 300K Solution, your samples are protected. Transport samples easily safely at room temperature and avoid the risks of cold chain logistics.
Two-Month Room Temperature Stability
No rush! Your RNA is safe for up to 2 months at room temperature, giving you flexibility without sacrificing sample quality.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality.
Versatile Applications!
Your RNA stays viable for any downstream appliations like RNAseq.
PRODUCTS
RNA aliquots extracted from peripheral blood were stored under two different conditions: frozen at -80 ºC (control) and freeze-dried at RT (22 ºC) with the 300K RNA Room Temperature Stabilization Solution.
RNA integrity analysis by Agilent 2200 TapeStation System.
RNA integrity was assessed using the Agilent 2200 TapeStation System. Analysis of RNA integrity numbers (RIN) in frozen and freeze-dried RNA samples after 1 week and 1, 2 and 8 months of storage showed no significant differences. These results confirm that the 300K RNA Stabilization Solution preserves high-quality RNA, with RIN values consistently greater than 7.
| Sample ID | Time of storage | Way of storage | RIN |
|---|---|---|---|
| RNA T1 | 1 week | -80 ºC | 7,90 |
| RT | 7,80 | ||
| RNA T2 | 1 month | -80 ºC | 7,90 |
| RT | 8,10 | ||
| RNA T3 | 2 months | -80 ºC | 8,20 |
| RT | 8,30 | ||
| RNA T4 | 8 months | -80ºC | 8,60 |
| RT | 8,50 |
To assess the suitability of the dried samples for genomic studies and notice if there were differences between both storage conditions through time, we performed 3’Tag RNA-Seq assay.
3'Tag RNA-Seq assay*
We assess RNA functionality using 3’Tag RNA-Seq assay of 6 samples from the same donor. 3 samples were frozen and 3 lyophilized.
Two cutoff points were used to distinguish between expressed and unexpressed genes, set at 100 (one per 10,000 total reads assigned to the gene) and 500 aligned reads for each gene (approximately 5 per 10,000 reads). The Upset plot shows the number of genes that are expressed in each sample combination (only combinations consistent with the experimental design are shown). As can be seen in the graph, most of the expressed genes appear in all samples, while the remaining combinations have a residual number of genes.
Differential expression analysis using EdgeR revealed no differentially expressed genes between frozen and lyophilized samples with an adjusted False Discovery Rate (FDR) of less than 0.05. These findings indicate that the 300K RNA Stabilization Solution effectively preserves RNA integrity, making freeze-dried RNA samples suitable for downstream applications.
| Sample ID | Time of storage | Way of storage | % (Aligned readings) | Number of readings |
|---|---|---|---|---|
| RNA T1 | 1 week | -80 ºC | 82,3 % | 1,6 M |
| RT | 81,9 % | 1,7 M | ||
| RNA T2 | 1 month | -80 ºC | 84,6 % | 1,3 M |
| RT | 82,3 % | 1,2 M | ||
| RNA T3 | 2 months | -80 ºC | 84,0 % | 1,5 M |
| RT | 81,2 % | 1,5 M | ||
| RNA T4 | 8 months | -80ºC | 87,7 % | 4,6 M |
| RT | 86,8 % | 4,6 M |
UPSET plot showing the genes expressed in common for each sample combination. More than 500 aligned reads were used as the expression criterion.
*We thank to the Bioinformatics Service of NUCLEUS, University of Salamanca, for the data analysis.
Check RNA resources:
