RNA ROOM TEMPERATURE STABILIZATION SOLUTION
Tired of worrying about your RNA degrading? Now you can stop stressing about unstable samples, 300K RNA Stabilization Solution keeps your RNA secure at room temperature, making storage and transport easier than ever!
Add security
RNA is fragile, but with the 300K Solution, your samples are protected. Transport samples easily safely at room temperature and avoid the risks of cold chain logistics.
Two-Month Room Temperature Stability
No rush! Your RNA is safe for up to 2 months at room temperature, giving you flexibility without sacrificing sample quality.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality.
Versatile Applications!
Your RNA stays viable for any downstream appliations like RNAseq.
PRODUCTS
RNA aliquots of 100 µL (50 ng/µL) extracted from peripheral blood were stored under two different conditions: frozen at -80 ºC (control) and freeze-dried at RT (22 ºC) with the 300K RNA Room Temperature Stabilization Solution.
RNA integrity analysis by Agilent 2200 TapeStation System.
RNA integrity was assessed using the Agilent 2200 TapeStation System. Analysis of RNA integrity numbers (RIN) in frozen and freeze-dried RNA samples after 1, 3 and 8 weeks of storage showed no significant differences. These results confirm that the 300K RNA Stabilization Solution preserves high-quality RNA, with RIN values consistently greater than 7.
| Sample ID | Time of storage | Way of storage | RIN |
|---|---|---|---|
| RNA T1 | 1 week | -80 ºC | 7,90 |
| RT | 7,80 | ||
| RNA T2 | 3 weeks | -80 ºC | 7,90 |
| RT | 8,10 | ||
| RNA T3 | 8 weeks | -80 ºC | 8,20 |
| RT | 8,30 | ||
| RNA T4 | 32 weeks | -80ºC | 8,60 |
| RT | 8,50 |
To assess the suitability of the dried samples for genomic studies and notice if there were differences between both storage conditions through time, we performed 3’Tag RNA-Seq assay.
3'Tag RNA-Seq assay
We assess RNA functionality using 3’Tag RNA-Seq assay. The results of this analysis showed no significant differences in the number of reads and the gene expression in both samples (stored at -80 ºC or RT) through time. These findings indicate that the 300K RNA Stabilization Solution effectively preserves RNA integrity, making freeze-dried RNA samples suitable for downstream applications.
| Sample ID | Time of storage | Way of storage | % (Aligned readings) | Number of readings |
|---|---|---|---|---|
| RNA T1 | 1 week | -80 ºC | 82,3 % | 1,6 M |
| RT | 81,9 % | 1,7 M | ||
| RNA T2 | 3 weeks | -80 ºC | 84,6 % | 1,3 M |
| RT | 82,3 % | 1,2 M | ||
| RNA T3 | 8 weeks | -80 ºC | 84,0 % | 1,5 M |
| RT | 81,2 % | 1,5 M |
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