RNA ROOM TEMPERATURE STABILIZATION SOLUTION
Tired of worrying about your RNA degrading? Now you can stop stressing about unstable samples, 300K RNA Stabilization Solution keeps your RNA secure at room temperature, making storage and transport easier than ever!
Add security
RNA is fragile, but with the 300K Solution, your samples are protected. Transport samples easily safely at room temperature and avoid the risks of cold chain logistics.
Ten-Month Room Temperature Stability
No rush! Your RNA is safe for up to 12 months at room temperature, giving you flexibility without sacrificing sample quality.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality.
Versatile Applications!
Your RNA stays viable for any downstream appliations like RNAseq.
PRODUCTS
HOW IT WORKS?
The 300K Room Temperature Stabilization Solution is a technology based on the lyophilization of biological samples in a uniform and standardized process. By removing nearly all moisture, it halts degradation processes and allows samples to be maintained at room temperature while preserving their integrity after rehydration. This process requires two key elements: the Sample Stabilization System (S³), a preset freeze-drying device developed by 300K to ensure optimal and reproducible conditions, and the Sample Stabilization Kits, which includes vials pre-coated with specific excipients that protect the sample during lyophilization without interfering in downstream analyses—so no washing is needed after rehydration. This combined system ensures reliable and ready-to-use stabilization, much more than a storage tube.
Stability of freeze-dried RNA at 12 Months
RNA aliquots extracted from peripheral blood were stored under two different conditions: frozen at -80 ºC (control) and freeze-dried at RT (22 ºC) with the 300K RNA Room Temperature Stabilization Solution. The following measurements demonstrate that RNA freeze-dried using 300K technology maintains its integrity and functionality up to 12 months at room temperature.
Feeze-dried RNA maintains RNA integrity numbers (RIN) above commonly accepted thresholds for transcriptomic applications such RNA-Seq (RIN ≥7.0) after 12 months of room temperature storage. Purity ratios remained within expected ranges, and RNA recovery following rehydration indicate no material loss.
| Sample | Ratio A260/280 | Ratio A260/230 | Concentration |
|---|---|---|---|
| Frozen 1 | 1,574 | 1.924 | 41.90 |
| Frozen 2 | 1.569 | 1.941 | 43.95 |
| Frozen 3 | 1.545 | 1.973 | 43.30 |
| Room Temperature 1 | 1.526 | 1.703 | 42.35 |
| Room Temperature 2 | 1.513 | 1.650 | 47.35 |
| Room Temperature 3 | 1.505 | 1.669 | 38.70 |
Table. Measurement of RNA purity with spectrophotometry (using Nanodrop OneC), RNA quantity with fluorimetry (using Qubit Flex) and RNA Integrity number (RIN) using Tapestation.
Figure. RNA integrity assessment using Agilent 2200 TapeStation System after different storage times. F: Frozen sample. RT: Room Temperature sample
Reverse transcription PCR (RT-PCR) targeting the ACTB housekeeping gene produced comparable amplification profiles between room-temperature stored and frozen samples, confirming that freeze-dried RNA remains
enzymatically accessible and suitable for reverse transcription and amplification reactions.
Figure. RT-PCR using ACTB gen. F: Frozen sample. RT: Room Temperature sample
To assess the suitability of the dried samples for genomic studies and notice if there were differences between both storage conditions through time, we performed 3’Tag RNA-Seq assay.
3'Tag RNA-Seq assay*
We assess RNA functionality using 3’Tag RNA-Seq assay of 6 samples from the same donor. 3 samples were frozen and 3 lyophilized.
Figure. Workflow for RNA functionality assessment using 3’Tag RNA-Seq assay.
Two cutoff points were used to distinguish between expressed and unexpressed genes, set at 100 (one per 10,000 total reads assigned to the gene) and 500 aligned reads for each gene (approximately 5 per 10,000 reads). The Upset plot shows the number of genes that are expressed in each sample combination (only combinations consistent with the experimental design are shown). As can be seen in the graph, most of the expressed genes appear in all samples, while the remaining combinations have a residual number of genes.
Differential expression analysis using EdgeR revealed no differentially expressed genes between frozen and lyophilized samples with an adjusted False Discovery Rate (FDR) of less than 0.05. These findings indicate that the 300K RNA Stabilization Solution effectively preserves RNA integrity, making freeze-dried RNA samples suitable for downstream applications.
| Time of storage | Way of storage | % (Aligned readings) | Number of readings |
|---|---|---|---|
| 1 week | -80 ºC | 82.3 % | 1.6 M |
| RT | 81.9 % | 1.7 M | |
| 1 month | -80 ºC | 84.6 % | 1.3 M |
| RT | 82.3 % | 1.2 M | |
| 2 months | -80 ºC | 84.0 % | 1.5 M |
| RT | 81.2 % | 1.5 M | |
| 8 months | -80ºC | 87.7 % | 4.6 M |
| RT | 86.8 % | 4.6 M |
Table. Percentage of aligned readings and total number of readings for frozen and lyophilized samples at different storage times.
Figure. UPSET plot showing the genes expressed in common for each sample combination. More than 500 aligned reads were used as the expression criterion.
*We thank to the Bioinformatics Service of NUCLEUS, University of Salamanca, for the data analysis.
