TISSUE ROOM TEMPERATURE STABILIZATION SOLUTION
Forget freezing and protect tissue samples the smarter way. 300K Tissue Stabilization Solution provides room temperature preservation without compromising molecular integrity, ensuring reliable results for genomic studies.
Perfect Preservation. No Freezing Needed!
Room temperature storage keeps your tissues ready for analysis—no freezers, no degradation risks.
Optimize your space
Store more samples in less space by keeping them at room temperature and free up valuable freezer space.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality.
PRODUCTS
HOW IT WORKS?
The 300K Room Temperature Stabilization Solution is a technology based on the lyophilization of biological samples in a uniform and standardized process. By removing nearly all moisture, it halts degradation processes and allows samples to be maintained at room temperature while preserving their integrity after rehydration. This process requires two key elements: the Sample Stabilization System (S³), a preset freeze-drying device developed by 300K to ensure optimal and reproducible conditions, and the Sample Stabilization Kits, which includes vials pre-coated with specific excipients that protect the sample during lyophilization without interfering in downstream analyses—so no washing is needed after rehydration. This combined system ensures reliable and ready-to-use stabilization, much more than a storage tube.
Coming soon…
Nucleic acids extraction for advanced genetics applications
To evaluate if our stabilization technology allowed long-term stability during RT storage of freeze-dried tissue samples, we compared DNA and RNA quality right after the freeze-drying process and after 5weeks of RT storage. This QC assessment is focussed on 2 different parameters: purity and integrity
First, we assessed DNA/RNA purity by spectrophotometry showing a A260/280 ratio values above 1.6 even after 5 weeks of RT storage, which indicates absence of massive protein contamination.
DNA purity
| Tissue | A260/280 Control (T0) | A260/280 5 weeks |
|---|---|---|
| Lung | 2.05 | 2.07 |
| Kidney | 2.10 | 2.08 |
| Liver | 2.11 | 2.09 |
Table. DNA purity (A260/280 ratio) in lung, kidney, and liver tissues after freeze-drying and 5 weeks of RT storage.
RNA purity
| Tissue | A260/280 Control (T0) | A260/280 5 weeks |
|---|---|---|
| Lung | 1.85 | 1.93 |
| Kidney | 2.01 | 2.05 |
| Liver | 1.96 | 1.98 |
Table. RNA purity (A260/280 ratio) in lung, kidney, and liver tissues after freeze-drying and 5 weeks of RT storage.
Second, using agarose gel electrophoresis we assessed DNA and RNA integrity in all samples.
DNA integrity
On one hand, regarding DNA integrity, we observed that despite the presence of a smear mostly in the control samples, a define band corresponding to high-weight DNA was detectable in all samples, even after 5 weeks of RT storage. Moreover, most of the DNA integrity values (DIN) were ≥6, confirming their good quality. Interestingly, only lung samples showed partial degradation as showed by their DIN values under 6.
Figure. Workflow for DNA integrity assessment in tissue samples after freeze-drying and RT storage.
Figure. DNA integrity values (DIN) and agarose gel electrophoresis results for lung, kidney, and liver tissues after 5 weeks of RT storage.
RNA integrity
On the other hand, regarding RNA integrity we observed define bands corresponding to 28S and 18S rRNA in all samples and conditions, even after 5 weeks of RT storage. Moreover, most of the RNA integrity values (RIN) were > 6, confirming their good quality.
Figure. Workflow for RNA integrity assessment in tissue samples after freeze-drying and RT storage.
Figure. RNA integrity values (RIN) and agarose gel electrophoresis results for lung, kidney, and liver tissues after 5 weeks of RT storage.
Immunohistochemistry
We assessed the suitability of our technology for histology and IHC analysis. First, we performed the commonly used hematoxylin & eosin (H&E) staining in lung sample, and observed that, although the tissue structure is largely preserved after lyophilization, it is also possible that this process leads to some areas with nuclear inclusions (red circle) similar to the alterations we can observe in non lyophilized samples when we have denser areas such as tumors.
Second, we performed IHC analysis using three widely used biomarker ssuch as Ki67, CD31 and Vimentin, and demonstrate that lyophilized tissue samples are suitable for this kind of technique
