Get rid of limitations associated with formalin fixation paraffin embedding storage, and store tissues at room temperature with 300K Tissue Solution adding safety and allowing the extraction of nucleic acids maintaining the same level of sample quality for downstream applications.
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300K Tissue Solution allows the use of RT stored tissues for immunohistochemistry analysis as shown by the detection of different highly used markers such as CD31, vimentin and Ki67.
QC of DNA extracted from samples dried with 300K Tissue Solution showed good purity and integrity when assessed by spectrophotometry and agarose gel electrophoresis, respectively.
Sample ID | A260/280 | A260/230 |
---|---|---|
-80 ºC lung | 1,92 | 2.25 |
Dried lung 1 | 1.91 | 2.25 |
Dried lung 2 | 1.96 | 2.30 |
-80 ºC kidney | 1,99 | 2.26 |
Dried kidney 1 | 1.98 | 2.32 |
Dried kidney 2 | 1.93 | 2.26 |
Likewise, QC of RNA extracted from samples dried with 300K Tissue Solution showed good purity and integrity when assessed by spectrophotometry (C) and agarose gel electrophoresis (D), respectively.
Sample ID (RNA) | A260/280 | A260/230 |
---|---|---|
-80 ºC lung | 1,97 | 2.31 |
Dried lung 1 | 1.88 | 2.4 |
Dried lung 2 | 1.85 | 2.37 |
-80 ºC kidney | 2,02 | 2.33 |
Dried kidney 1 | 2.01 | 2.30 |
Dried kidney 2 | 2 | 2.31 |
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