CENTRO NACIONAL DE INVESTIGACIONES ONCOLÓGICAS (CNIO)
A sustainable alternative to deep-freezing for plasma preservation.
Plasma is a complex biological fluid with a high protein content and significant clinical relevance. It is particularly sensitive to freeze-thaw cycles and storage conditions, which can affect the stability of its components and compromise downstream analyses.
Objective
To compare the quality and functionality of plasma samples stabilized and stored at room temperature vs. frozen at -80°C, in the context of a prospective collection of biobank samples.
Materials and Methods
Samples used for the study
Plasma obtained from peripheral blood in EDTA and citrate
30 samples
Fresh
Procedure
The collected samples are evaluated according to established quality control (QC) criteria before being divided into four fractions: two for lyophilization and two for freezing at -80°C. Aliquots of 500 µL are prepared for lyophilization, which is performed on the same day and concurrently with the freezing of parallel aliquots. All lyophilized samples are stored at room temperature.
The quality of fresh samples will be analyzed prior to lyophilization, while the stability of both frozen and lyophilized samples will be evaluated after 1 and 6 months of storage.
Quality Assurance
Electrophoresis followed by Coomassie staining
ELISA
Quality control will include ELISA test and polyacrylamide gel electrophoresis followed by Coomassie staining to analyze the protein profile. The signal intensity of the predominant bands obtained in the gels will be quantified, allowing an accurate assessment of protein integrity and stability under each storage condition.
Results
Coming soon…
Conclusion
Coming soon…
