Hospital de salamanca
A sustainable alternative to deep-freezing for plasma preservation.
Plasma is a complex biological fluid with a high protein content and significant clinical relevance. It is particularly sensitive to freeze-thaw cycles and storage conditions, which can affect the stability of its components and compromise downstream analyses.
Objective
To compare the quality and functionality of plasma samples stabilised and stored at room temperature vs. frozen at -80ºC, in the context of a prospective collection of biobank samples. The quality of freeze-dried samples will be compared to frozen samples to demonstrate their usefulness in studying the bone marrow microenvironment of patients diagnosed with MDS compared to healthy donors.
Materials and Methods
Samples used for the study
Plasma samples
15-20 samples
Fresh
Procedure
The collected samples shall be evaluated according to the QA and subsequently divided into 5 parts: 1 for initial control, 2 for freeze-drying and 2 for freezing at 80°C. Aliquots of 500 µL shall be lyophilised immediately after collection and at the same time as freezing. All freeze-dried samples, will be stored at room temperature .
Lyophilized samples will be compared to frozen samples using defined quality criteria to demonstrate their utility in studying the bone marrow microenvironment of patients diagnosed with MDS compared to healthy donors.
Samples are evaluated at three different times: first, fresh, immediately after collection to perform an initial quality control (QC) corresponding to time 0; then, after one month of lyophilized or frozen storage (time: 1 month); and finally, after six months of storage under the same conditions (time: 6 months).
Quality Assurance
LXSAHM-22 Human Luminex Discovery Assay
In MDS, the relationship between bone marrow cells and changes in the bone marrow niche are altered, leading to increased apoptosis, increased inflammation, reduced red blood cell production, reduced haematopoiesis and bone marrow senescence, all of which may contribute to the development of MDS. All of these changes lead to a pro-inflammatory microenvironment in the BM that drives proliferation and may induce clonal selection of stem cells. A cytokine panel will be used to compare the two techniques.
Results
Coming soon…
Conclusion
Coming soon…
