Hospital puerta de hierro
A sustainable alternative to deep-freezing for Plasma preservation.
Plasma is a complex biological fluid with a high protein content and significant clinical relevance. It is particularly sensitive to freeze-thaw cycles and storage conditions, which can affect the stability of its components and compromise downstream analyses.
Objective
To compare the quality and functionality of plasma samples stabilized and stored at room temperature versus those frozen at -80°C as part of a prospective biobank sample collection.
Materials and Methods
Samples used for the study
Plasma samples
30 samples
Fresh
Procedure
The samples collected will be evaluated according to the QC established in the following section and subsequently divided into 5 parts: 1 for initial control, 2 for lyophilization and 2 for freezing at 80ºC. Aliquots of 500 µL will be lyophilized immediately after collection and at the same time as freezing.
All lyophilized samples, once the lyophilization cycle is completed, and after verifying that the pellet has been formed correctly, will be stored at room temperature in the corresponding boxes.
After a period of one and six months during which the samples were stored in a desiccated state, a second evaluation of the freeze-dried samples was conducted, with a comparison made to the frozen samples.
Quality Assurance
IL8, IFN-gamma, TNF-alpha, IL-6, IL-10
Simultaneous detection and quantification of IL8, IFN-gamma, TNF-alpha, IL-6, IL-10 secreted in serum, using Luminex technology.
Results
Coming soon…
Conclusion
Coming soon…
