CELL LINES ROOM TEMPERATURE STABILIZATION SOLUTION
Say goodbye to freezers and discover a smarter way to preserve cell lines. 300K Cell Line Stabilization Solution ensures unmatched reliability and efficiency for your genomic research.
Perfect Preservation. No Freezing Needed!
Room temperature storage keeps your cell lines intact and ready for analysis—no freezers, no degradation risks.
Reference Material
Improve standarization and achive reproducible results by creating reference materials at room temperature.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality
PRODUCTS
The DNA extracted from samples freeze-dried using 300K Cell Lines Solution has been subjected to rigorous quality control to assess DNA purity, integrity and functionality room temperature storage, following the Proficiency Standards stablished by the International Society for Biological and Environmental Repositories (ISBER).
DNA purity and yield
| Sample ID | Time of RT storage | DNA yield (µg) | A260/280 |
|---|---|---|---|
| H929 | Control (T0) | 1,65 | 1,99 |
| 3 months | 2,76 | 1,93 | |
| CA46 | Control (T0) | 1,42 | 1,97 |
| 3 months | 2,59 | 1,88 | |
| RS4;11 | Control (T0) | 0,52 | 1,92 |
| 3 months | 0,94 | 1,81 | |
| REH | Control (T0) | 2,97 | 1,93 |
| 3 months | 1,75 | 1,86 |
DNA Functionality and integrity
To assess DNA functionality and integrity, we performed a multiplex long PCR, and a 17,5 kb band was observed in all four cell lines, even after 3 months of RT storage.
Suitability for NGS studies
Specifically, we performed the EuroClonality-NDC assay (Univ8 Genomics, Belfast, North Ireland) which is used for the study of Lymphoproliferative disorders (LPD). The reason why we choose this genomic assay is because it uses as validation samples, among others, the four cell lines we have tested.
The metrics obtained in this NGS analysis using DNA from all four cell lines (stored 1 year at RT) were within the stablished acceptance criteria. Moreover, the results (shown in the table below) were the expected for these specific cell lines (results regarding SNVs, even though not shown, were also similar in all four cell lines).
| Cell Line | Expected Translocation | Detected |
|---|---|---|
| CA46 | t(8;14) MYC/IGHswc | Yes |
| H929 | t(4;14) MMSET/IGHswc | Yes |
| H929 | t(8;20) MYC/FAM242A | Yes |
| REH | t(1;2) HYDIN2/IGKV | Yes |
| RS4;11 | t(4;11) AFF1/KMT2A | Yes |
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