CELL LINES ROOM TEMPERATURE STORAGE SOLUTION
Get rid of constrictions associated with cell culture and maintenance, and store cell line aliquots at room temperature with 300K Cell Line solution adding safety and allowing the extraction of DNA maintaining the same level of sample quality for downstream applications.
- Stabilization buffer to protect cell integrity from room temperature associated DNA degradation
- 24 vials and stoppers
- Use aliquots from 5 x 105 to 107 cells.
- Final volume of 250 µL
The DNA extracted from samples freeze-dried using 300K Cell Lines Solution has been subjected to rigorous quality control to assess DNA purity, integrity and functionality RT storage, following the Proficiency Standards stablished by the International Society for Biological and Environmental Repositories (ISBER).
- DNA purity and yield
Using DNA extracted from 4 different lymphoproliferative disorders (LPDs) cell lines (H929, CA46, RS4;11 and REH) DNA purity was assessed by spectrophotometry revealing that all four cell lines (aliquots of 5 x 105 cells) showed A260/280 ratio values above 1.6 even after 3 months of RT storage, which indicates absence of protein contamination. Moreover, double-strand DNA quantification by fluorimetry confirmed a good DNA yield in all samples (at least 0,5 µg).
|Sample ID||Time of RT storage||DNA yield (µg)||A260/280|
- DNA Functionality and integrity
To assess DNA functionality and integrity, we performed a multiplex long PCR, and a 17.5 kb band was observed in all four cell lines, even after 3 months of RT storage.
Suitability for NGS studies:
Specifically, we performed the EuroClonality-NDC assay (Univ8 Genomics, Belfast, North Ireland) which is used for the study of Lymphoproliferative disorders (LPD). The reason why we choose this genomic assay is because it uses as validation samples, among others, the four cell lines we have tested.
The metrics obtained in this NGS analysis using DNA from the H929 cell line (aliquots of 106 cells) were within the stablished acceptance criteria. Moreover, the results (shown in the table below) were the expected for this specific cell line.
Poster: «Use of Freeze-Dried Cell Lines as Controls for Next-Generation Sequencing Studies»