CELL ROOM TEMPERATURE STABILIZATION SOLUTION
Discover a smarter way to preserve cells at room temperature and say goodbye to the -80ºC freezers. 300K Cell Stabilization Solution ensures unmatched reliability and efficiency for your genomic research. It is optimize for White Blood Cells , Buffy coat and Cell lines.
Perfect Preservation. No Freezing Needed!
Room temperature storage keeps cells DNA intact and ready for analysis—no freezers, no degradation risks.
Optimize your space
Store more samples in less space by keeping them at room temperature and free up valuable freezer space.
Save on Operational Costs
Reduce refrigeration expenses and maintenance. Cut costs while maintaining quality
Versatile Applications
Applicable for white blood cells, buffy coat and cell lines. DNA remains viable for high-level analyses.
Shipping has never been this simple.
Transport samples easily safely at room temperature and avoid the risks of cold chain logistics.
Reference Material
Improve standardisation and achieve reproducible results by using cell lines to create reference materials at room temperature.
PRODUCTS
The DNA extracted from samples freeze-dried using 300K Cell Lines Solution has been subjected to rigorous quality control to assess DNA purity, integrity and functionality room temperature storage, following the Proficiency Standards stablished by the International Society for Biological and Environmental Repositories (ISBER).
DNA purity and yield
| Sample ID | Time of RT storage | DNA yield (µg) | A260/280 |
|---|---|---|---|
| H929 | Control (T0) | 1,65 | 1,99 |
| 3 months | 2,76 | 1,93 | |
| CA46 | Control (T0) | 1,42 | 1,97 |
| 3 months | 2,59 | 1,88 | |
| RS4;11 | Control (T0) | 0,52 | 1,92 |
| 3 months | 0,94 | 1,81 | |
| REH | Control (T0) | 2,97 | 1,93 |
| 3 months | 1,75 | 1,86 |
DNA Functionality and integrity
Using agarose gel electrophoresis, we confirmed DNA integrity in all four cell lines (A), which we further assessed together with DNA functionality, by multiplex long PCR. All samples displayed a 17.5 kb band (B), even after 3 months of RT storage.
At 300K Solutions, we validate the compatibility of our stabilized samples with different downstream applications.
Suitability for NGS studies
Specifically, we performed the EuroClonality-NDC assay (Univ8 Genomics, Belfast, North Ireland) which is used for the study of Lymphoproliferative disorders (LPD). The reason why we choose this genomic assay is because it uses as validation samples, among others, the four cell lines we have tested.
The metrics obtained in this NGS analysis using DNA from all four cell lines (stored 1 year at RT) were within the stablished acceptance criteria. Moreover, the results (shown in the table below) were the expected for these specific cell lines (results regarding SNVs, even though not shown, were also similar in all four cell lines).
| Cell Line | Expected Translocation | Detected |
|---|---|---|
| CA46 | t(8;14) MYC/IGHswc | Yes |
| H929 | t(4;14) MMSET/IGHswc | Yes |
| H929 | t(8;20) MYC/FAM242A | Yes |
| REH | t(1;2) HYDIN2/IGKV | Yes |
| RS4;11 | t(4;11) AFF1/KMT2A | Yes |
Valid for Allele Specific Real-Time Quantitative PCR (ASO-qPCR)
We performed a study with the KIT D816V mutation, characteristic of the mastocytosis cell line HMC1, comparing one fresh sample with three lyophilized samples. Dilutions were prepared to obtain a 10% frequency of the mutation, and since the mutation is present in only one allele, the expected frequency of detection was 5%.
The results showed that the fresh sample had 5.01% mutated cells, while the lyophilized samples gave values of 5,22%, 5,40% and 6,65%, with a deviation of 30% (range 3,5% – 6,5%) considered acceptable. This confirmed that 2 of the 3 lyophilized samples were within the expected limits, demonstrating that our stabilization technology allows samples to be stored at room temperature without compromising the accuracy of genetic analyses and ensuring their reliability in downstream applications such as ASO-qPCR.
| Sample | Result | Mutant cells |
|---|---|---|
| Fresh | Positive | 5,01% |
| Lyo. 1 | Positive | 5,22% |
| Lyo. 2 | Positive | 5,40% |
| Lyo. 3 | Positive | 6,65% |
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