FAQs
GENERAL QUESTIONS
Our solution is based on the process of freeze-drying, adapted to the process in research centres, hospitals, laboratories, or any company working with biological samples. Thanks to a series of patented technological innovations in our S³ device and stabilization kits, designed for each type of biospecimen, we have been able to adapt and standardize this process.
Our Stabilization Solution for DNA, RNA, PLASMA&SERUM and PERIPHERAL BLOOD is already available for users. Currently, we are developing solutions for other types of samples, such as cells and tissues, which will be available soon.
Stabilized samples can be stored in a conventional shelving cabinet maintained at room temperature (between 15°C and 27°C), and protected from direct exposure to sunlight.
Yes. Besides of minimizing the sample loss risk due to failures in the ULT or delays in dry ice shipping, in 300K Solutions, we conduct quality controls for each of our applications. You can check the quality controls we have performed for:
Our solution is ready-to-use and has been designed for easy integration into the user’s regular workflow. In general terms, a protocol would include the following steps:
Yes. Just as you would with cryopreserved samples, samples stabilized with our solution can be used and stabilized again following the corresponding SOPs for each application included in the stabilization kits.
No. The specific excipient formulations for each biological sample have been carefully designed to not interfere in downstream applications.
DNA
We have tested the purity, integrity and functionality of samples stabilized with our solution for 5 years at room temperature (22ºC) and at 60°C (accelerated degradation conditions).
Our solution allows stabilizing from 100 to 400µL of purified DNA with concentrations ranging from 25 to 200 ng/µL (DNA stabilization kit 24).
In case of having a volume less than 100µL. It is recommended to adjust it to 100µL volume using the elution buffer you used in your extraction. It is important to note that by adjusting the volume, the final DNA concentration will change.
The S3 device can process two SBS racks at the same time. This means that in one cycle, you can stabilize either 48 samples using the DNA Stabilization Kit 24 or 96 samples using the DNA Stabilization Kit 48.
For rehydration, add Biology Grade Water to the same volume as that of the original lyophilized material.
Yes. You can re-stabilize the leftover sample if you have 200µL or more left. You simply need to select the corresponding program on the device and load the vial(s) and repeat the program just as you did the first time.
To use the re-stabilized sample, you will need to rehydrate them with the same volume of molecular biology-grade water.
We have performed the process up to 20 times providing good results.
There is no minimum number of samples that can be stabilized per run.
If you prefer to accumulate all the samples received over time, we recommend to aliquot them into our vials first and then keep these samples frozen at -20ºC (up to 1 month) and proceed with the normal protocol once you decide to stabilize them at room temperature.
300K Solutions has subjected DNA samples to quality control based on the standards of the Spanish National DNA Bank and the ISBER Best Practices for Repositories guidelines:
- Agarose gel electrophoresis to study integrity
- Spectrophotometry to study purity and concentration
- Long multiplex PCR to study functionality and integrity
- Qubit fluorometer to study concentration
RNA
We have test the integrity of RNA samples stabilized with our solution for 8 months at room temperature.
Our solution allows stabilizing 50µL of purified RNA with a maximum concentration of 50 ng/µL. (RNA stabilization kit 48).
In case of having a volume less than 50µL, the solution would be to adjust to 50µL volume using the elution buffer you used in your extraction. It is important to note that by adjusting the volume, your final RNA concentration will change.
Our S3 equipment can accommodate two racks in a single cycle. Therefore, in one cycle you can stabilize 96 samples.
RNA Stabilization Kit 48: with 48 vials coated with lyobuffer.
For rehydration, add Biology Grade Water to the same volume as that of the original lyophilized material.
There is no minimum number of samples that can be stabilized per run.
300K Solutions has subjected RNA samples to quality control based on the standards of the Spanish National DNA Bank and the ISBER Best Practices for Repositories guidelines:
- RNA integrity analysis by TapeStation System
PLASMA & SERUM
Our solution allows for stabilizing from 500 to 1000 µL of plasma in our Plasma and Serum Stabilization kit 24
Our S³ device can accommodate two racks in a single cycle. Therefore, in one cycle you can stabilize 48 samples of Plasma and Serum Stabilization Kit 24
For rehydration, add Biology Grade Water to the same volume as that of the original lyophilized material.
Yes. You can re-stabilize the leftover sample if you have 200µL or more left. You simply need to select the corresponding program on the device and load the vial(s) and repeat the program just as you did the first time.
To use the re-stabilized sample, you will need to rehydrate them with the same volume of molecular biology-grade water.
There is no minimum number of samples that can be stabilized per run.
If you prefer to accumulate all the samples received over time, we recommend to aliquot them into our vials first and then keep these samples frozen at -20ºC (up to 1 month) and proceed with the normal protocol once you decide to stabilize them at room temperature.
