FLOW CYTOMETRY ALIGNMENT PARTICLES
A wide variety of particles for determining if a flow cytometer is properly aligned and has a clean flow cell with no fluidic blockage. Using SPHERO Alignment Particles, the coefficients of variation (CVs), peak channels, and histogram distributions can be measured to determine the alignment and functionality of the flow cytometer.
Additional information can be found at STN-8: Calibration and Performance Tracking of Flow Cytometer Using SPHERO Calibration Particles
SPHERO Ultra Rainbow Fluorescent Particles
– A single population of Rainbow Fluorescent Particles with enhanced UV and Far Red fluorescence intensity.
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
|---|---|---|---|---|
| URFP-02-2 | Ultra Rainbow Fluorescent Particles | 1010/mL | 0.1-0.3 µm | 2 mL |
| URFP-05-2 | Ultra Rainbow Fluorescent Particles | 107/mL | 0.4-0.6 µm | 2 mL |
| URFP-10-5 | Ultra Rainbow Fluorescent Particles | 107/mL | 1.0-1.4 µm | 5 mL |
| URFP-30-2 | Ultra Rainbow Fluorescent Particles | 107/mL | 3.0-3.4 µm | 2 mL |
| URFP-30-20 | Ultra Rainbow Fluorescent Particles | 107/mL | 3.0-3.4 µm | 20 mL |
| URFP01-30-2K | Ultra Rainbow Fluorescent Particles | 106/mL | 3.0-3.4 µm | 2x15 mL |
| URFP01-30-10K | Ultra Rainbow Fluorescent Particles | 106/mL | 3.0-3.4 µm | 10x3 mL |
| URFP-38-2 | Ultra Rainbow Fluorescent Particles | 107/mL | 3.5-3.9 µm | 2 mL |
| URFP-38-5A | Ultra Rainbow Fluorescent Particles, Mid-Range | 107/mL | 3.5-3.9 µm | 5 mL |
| URFP-100-2 | Ultra Rainbow Fluorescent Particles | 107/mL | 8.1-12.0 µm | 2 mL |
| URFP-150-2 | Ultra Rainbow Fluorescent Particles | 5x106/mL | 13.0-17.9 µm | 2 mL |
| URFP-200-5 | Ultra Rainbow Fluorescent Particles | 1.0% w/v | 18.0-24.9 µm | 5 mL |
| URFP-300-5 | Ultra Rainbow Fluorescent Particles | 1.0% w/v | 25.0-35.0 µm | 5 mL |
SPHERO Rainbow Fluorescent Particles
– A single population of Rainbow Fluorescent Particles for the alignment of FITC, PE, PE-TR, and PE-Cy5 channels.
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
|---|---|---|---|---|
| RFP-20-5 | Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-20-5) | 107/mL | 1.8-2.2 µm | 5 mL |
| RFP-30-5 (1 peak) | Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-30-5) | 107/mL | 3.0-3.5 µm | 5 mL |
| RFP-30-5A (1 peak) | Rainbow Fluorescent Particles (Intensity similar to mid range FL1 fluorescence in RCP-30-5) | 107/mL | 3.0-3.4 µm | 5 mL |
| RFP-35-5 | Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-35-5) | 107/mL | 3.5-4.0 µm | 5 mL |
| RFP-50-5 | Rainbow Fluorescent Particles | 107/mL | 5.0-5.9 µm | 5 mL |
| RFP-60-5 | Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-60-5) (Recommended for FL1, FL2, and FL3 channels only) | 107/mL | 6.0-6.4 µm | 5 mL |
| RFP-70-5 | Rainbow Fluorescent Particles | 107/mL | 6.5-8.0 µm | 5 mL |
| RFP-100-2 | Rainbow Fluorescent Particles | 107/mL | 8.1-12.0 µm | 2 mL |
SPHERO Supra Rainbow Midrange Fluorescent Particles
– Consists of a single population which has an intensity similar to cellular samples with excellect CVs in all channels.
– Excellent for instrument set-up at the same fluorescent settings as the analysis of cellular samples.
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
|---|---|---|---|---|
| SRCP-35-2A | Supra Rainbow Midrange Fluorescent Particles | 107/mL | 3.0-3.59 µm | 2 mL |
| SRCP-35-5A | Supra Rainbow Midrange Fluorescent Particles | 107/mL | 3.0-3.59 µm | 5 mL |
| SRCP01-35-10A | Supra Rainbow Midrange Fluorescent Particles | 106/mL | 3.0-3.59 µm | 10 mL |
SPHERO Fluorescent Alignment Particles
– Inexpensive, highly concentrated of Fluorescent Particles for the alignment of FITC, PE, PE-TR, and PE-Cy5 channels.
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
|---|---|---|---|---|
| FAP-3056-5 | Fluorescent Alignment Particles | 108/mL | 3.0-3.4 µm | 5 mL |
SPHERO Ultra Rainbow Fluorescent Particles for Beckman Coulter Cytometers
– Consists of a single peak for performance verification of Beckman Coulter flow cytometers.
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
|---|---|---|---|---|
| C62930 | Calibration Beads for MoFlo Astrios | 107/mL | 3.0-3.4 µm | 2 mL |
| B28479 | Calibration Beads for MoFlo, MoFlo XDP & Cyan ADP | 107/mL | 3.0-3.4µm | 2 mL |
| ACURFP-38-5 | AccuCount Ultra Rainbow Fluorescent Particles (Calibration beads for Aquios CL) | 106/mL | 3.8 µm (+/-0.3) | 5 mL |
| ACURFP-38-15 | AccuCount Ultra Rainbow Fluorescent Particles (Calibration beads for Aquios CL) | 106/mL | 3.8 µm (+/-0.3) | 15 mL |
SPHERO Rainbow Alignment Particles
– Prediluted Rainbow Fluorescent Particles with enhanced UV and Far Red fluorescence intensity.
| Catalog No. | Description | Concentration | Nominal Size | Package Size |
|---|---|---|---|---|
| RAP-38-5 | Rainbow Alignment Particles | 1.2x106/mL | 3.5-3.9 µm | 5 mL |
